An electron beam welding machine comprises an electron gun including a first beam adjusting means, a second beam adjusting means disposed spaced from the first beam adjusting means so as to be movable in the direction of an electron beam path, and position adjusting means for adjusting the position of the second beam adjusting means through rotary movement about an axis perpendicularly crossing the electron beam path and linear movements on a plane perpendicular to the electron beam path. The machine further includes a mirror and light source mounted on the electron gun to emit light on the electron beam path, and photodetectors mounted on the opposite sides of the second beam adjusting means so as to face the electron gun and a workpiece, whereby the correct position of the second beam adjusting means is detected.