发明授权
- 专利标题: System for determining the concentration of an L-amino acid in fermentation
- 专利标题(中): 确定发酵中L-氨基酸浓度的系统
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申请号: US237668申请日: 1981-02-24
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公开(公告)号: US4384936A公开(公告)日: 1983-05-24
- 发明人: Haruo Obana , Tadashi Shirakawa , Motohiko Hikuma , Takeo Yasuda , Isao Karube , Shuichi Suzuki
- 申请人: Haruo Obana , Tadashi Shirakawa , Motohiko Hikuma , Takeo Yasuda , Isao Karube , Shuichi Suzuki
- 申请人地址: JPX Tokyo
- 专利权人: Ajinomoto Company, Incorporated
- 当前专利权人: Ajinomoto Company, Incorporated
- 当前专利权人地址: JPX Tokyo
- 优先权: JPX53-35906 19780328; JPX53-65380 19780531
- 主分类号: C12Q1/00
- IPC分类号: C12Q1/00 ; C12Q1/02 ; G01N33/487 ; C12M1/40
摘要:
Electrochemical method for determining the concentration of a carbon source and L-amino acid in a fermentation medium or cultured broth by contacting a microbial electrode consisting of an oxygen-sensitive electrode or a carbon dioxide gas-sensitive electrode combined with fixed microbial cells of a microorganism capable of assimilating said carbon source or decarboxylating said L-amino acid with a sample solution and sensing electrically the rate of current decrease which is caused by the consumed oxygen in the solution in proportion to the concentration of carbon source; or the electric motive force which is caused by the liberation of carbon dioxide in the sample solution in proportion to the concentration of L-amino acid. Microbial electrodes and systems useful in carrying out the aforementioned method are disclosed.