发明授权
US4401759A Detection and isolation of glucagon mRNA using a synthetic
oligodeoxynucleotide
失效
使用合成的寡脱氧核苷酸检测和分离胰高血糖素mRNA
- 专利标题： Detection and isolation of glucagon mRNA using a synthetic oligodeoxynucleotide
- 专利标题（中）： 使用合成的寡脱氧核苷酸检测和分离胰高血糖素mRNA
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申请号： US366593申请日： 1982-04-08
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公开(公告)号： US4401759A公开(公告)日： 1983-08-30
- 发明人: Harvey Rubin , Richard H. Tullis
- 申请人： Harvey Rubin , Richard H. Tullis
- 专利权人： Harvey Rubin,Tullis Richard H
- 当前专利权人： Harvey Rubin,Tullis Richard H
- 主分类号： C07K14/605
- IPC分类号： C07K14/605 ; C12Q1/68 ; C12N15/00 ; C12P19/34 ; C12P21/00
摘要：
A synthetic oligodeoxynucleotide complementary to glucagon mRNA and a method of using it to detect and isolate glucagon mRNA and cDNA from human and rabbit pancreas. A unique 14 base oligodeoxynucleotide dTTCATCAGCCACTG wherein T represents thymine, G represents guanine, A represents adenine, and C represents cytosine and where at least 13 of the 14 nucleotides are as indicated (one of those indicated may be replaced by one of the other three mentioned nucleotides) has been found to be complementary to glucagon mRNA. To isolate glucagon mRNA, total RNA is first extracted from human or rabbit pancreas and A+ RNA (mRNA-poly A) is isolated from the total RNA. The A+ RNA is then treated with the oligodeoxynucleotide, and the resulting hybridized RNA is enzymatically converted to glucagon mRNA which can then be purified, copied into glucagon cDNA and used in a conventional manner to produce glucagon by cloning technique.
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