发明授权
- 专利标题: Process for obtaining insulin precursors from reaction mixtures resulting from the folding of insulin precursors from the corresponding S-sulfonates
- 专利标题(中): 从由相应的S-磺酸盐折叠胰岛素前体产生的反应混合物获得胰岛素前体的方法
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申请号: US924850申请日: 1986-09-02
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公开(公告)号: US4801684A公开(公告)日: 1989-01-31
- 发明人: Ulrich Grau
- 申请人: Ulrich Grau
- 申请人地址: DEX Frankfurt am Main
- 专利权人: Hoechst Aktiengesellschaft
- 当前专利权人: Hoechst Aktiengesellschaft
- 当前专利权人地址: DEX Frankfurt am Main
- 优先权: DEX3501641 19850119
- 主分类号: C07K1/30
- IPC分类号: C07K1/30 ; C07K1/107 ; C07K1/113 ; C07K14/575 ; C07K14/62 ; C07K14/625 ; C07K7/40
摘要:
A process for the preparation of insulin precursors of the formula (I) ##STR1## in which R is hydrogen or the presequence P.sub.m --Q.sub.n --, in which P is a sequence of naturally occurring amino acids with m being from 0 to 50, Q are basic natural amino acids, and n is an integer from 1 to 4, Y represents --Lys.sup.B29 --Z.sup.B30 --, in which Z denotes Ala, Thr or Ser, and the bridge extending from A-1 to A-21 is an insulin A-chain, the bridge extending from B-1 to B-30 represents an insulin B-chain, and X is a bridge which is bonded to the insulin A-chain at the amino group of A-1 and is bonded to the insulin B-chain at the .epsilon.-amino group of B-29-- in which case it is bonded to the free bond of Z.sup.B30 OH-- or at the carboxyl group of B-30, from reaction mixtures which result from folding of insulin precursors from S-sulfonates of the formula (II) ##STR2## in which R, X and Y have the abovementioned meanings, which comprises the false recombinants contained in the reaction mixture being precipitated by adjustment of the reaction mixture to pH 4 to 6, the precipitate being removed in customary manner and converted by sulfitolysis into the S-sulfonate of the formula (II), and the latter then being subjected to renewed folding.