发明授权
- 专利标题: Method for the determination of the reduced form of nicotinamide adenine dinucleotide
- 专利标题(中): 测定烟酰胺腺嘌呤二核苷酸还原形式的方法
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申请号: US706404申请日: 1985-02-28
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公开(公告)号: US4824779A公开(公告)日: 1989-04-25
- 发明人: Norihito Aoyama , Akira Miike , Yoshiaki Shimizu , Toshio Tatano
- 申请人: Norihito Aoyama , Akira Miike , Yoshiaki Shimizu , Toshio Tatano
- 申请人地址: JPX Tokyo
- 专利权人: Kyowa Medex Co., Ltd.
- 当前专利权人: Kyowa Medex Co., Ltd.
- 当前专利权人地址: JPX Tokyo
- 优先权: JPX59-37623 19840229
- 主分类号: G01N33/50
- IPC分类号: G01N33/50 ; C12Q1/00 ; C12Q1/26 ; C12Q1/28
摘要:
The present invention provides a method for the determination of NAD(P)H which comprises reacting a chromogen represented by the following general formula (I): ##STR1## wherein Y is hydrogen or hydroxyl; R.sub.1, R.sub.2 and R.sub.3 may be the same or different, and are groups represented by the following general formula (II), (III) or (IV): ##STR2## wherein Z is hydrogen, hydroxyl, amino, substituted amino, alkyl, substituted alkyl, alkenyl, aryl, substituted aryl, acyl, halogen, nitro, sulfo, carboxyl or alkoxy; n is 1, 2, or 3; provided that at least one Z in R.sub.1, R.sub.2 and R.sub.3 is hydroxyl, amino, or substituted amino; and Z.sub.s in Z.sub.n may be the same or different, with NAD(P)H in the presence of (1) peroxidase or thiol oxide reductase and (2) diaphorase or an electron carrier, and determining the pigment thus formed.