发明授权
- 专利标题： Plasmid, method for construction of the same, microorganisms carrying the plasmid and method for cultivation of the microorganism
- 专利标题（中）： 质粒，其构建方法，携带质粒的微生物和培养微生物的方法
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申请号： US32032申请日： 1987-03-30
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公开(公告)号： US4962055A公开(公告)日： 1990-10-09
- 发明人: Koki Horikoshi , Toshiaki Kudo , Chiaki Kato
- 申请人： Koki Horikoshi , Toshiaki Kudo , Chiaki Kato
- 申请人地址： JPX Wako
- 专利权人： Rikagaku Kenkyusho
- 当前专利权人： Rikagaku Kenkyusho
- 当前专利权人地址： JPX Wako
- 优先权： JPX58-38087 19830308; JPX58-38089 19830308
- 主分类号： C12N1/21
- IPC分类号： C12N1/21 ; C12N1/38 ; C12N9/24 ; C12N9/86 ; C12N15/70
摘要：
A novel plasmid pEAP2, which was constructed from Bacillus sp. 170 chromosomal DNA carrying the gene for extracellular production of penicillinase and a vector plasmid pMB9. A novel microorganism, Escherichia coli HB101(pEAP2) carrying the plasmid pEAP2 and being capable of extracellular production of penicillinase. The method for cultivation of the microorganism is characterized by culturing it in the medium containing NaCl (or KCl) for 16-48 hours. According to this invention, useful high-molecular substances can be produced in a high yield.
公开/授权文献
- US5906145A Roofing shovel 公开/授权日：1999-05-25
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