A sewing machine for automatic thread take-up and threading, which comprises a guide groove for guiding a needle thread from a bobbin to a threading preparatory position via a thread take-up preparatory position, a sewing machine motor driver and a sewing machine motor for extracting the needle thread at the thread take-up preparatory position along the thread take-up moving area and for taking up the needle thread using a thread receiver and/or a thread take-up, and a thread positioning member for keeping the end of the needle thread guided to the threading preparatory position and for positioning the needle thread in front of or at the rear of the eye of a needle, a threading member for performing the threading operation of the needle thread, and a prohibiting member for prohibiting the threading operation until the thread take-up operation is over. In the sewing machine, the thread take-up operation and the heading operation are automatically and successively performed. The needle thread is kept at its end by a thread positioning member and a tension member is opened during the thread take-up operation. Therefore, the end of the needle thread is not drawn toward the thread take-up. The operator has only to put the needle thread along the guide groove and does not have to prepare the needle thread twice for the thread take-up operation and the threading operation.