发明授权
US5089398A Enhanced yeast transcription employing hybrid GAPDH promoter region
constructs
失效
使用混合GAPDH启动子区构建体增强酵母转录
- 专利标题： Enhanced yeast transcription employing hybrid GAPDH promoter region constructs
- 专利标题（中）： 使用混合GAPDH启动子区构建体增强酵母转录
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申请号： US380783申请日： 1989-07-18
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公开(公告)号： US5089398A公开(公告)日： 1992-02-18
- 发明人: Steven Rosenberg , Patricia Tekamp-Olson
- 申请人： Steven Rosenberg , Patricia Tekamp-Olson
- 申请人地址： CA Emeryville
- 专利权人： Chiron Corporation
- 当前专利权人： Chiron Corporation
- 当前专利权人地址： CA Emeryville
- 主分类号： C07K14/02
- IPC分类号： C07K14/02 ; C07K14/81 ; C12N9/02 ; C12N15/81
摘要：
Yeast promoters of glycolytic enzymes are modified by isolating a fragment encompassing the RNA polymerase binding site and joining to the 5' end of this fragment a DNA sequence providing for enhanced inducible or constitutive transcription of a structural gene. Constructs are prepared for efficient expression of foreign genes in yeast.Yeast strains 2150-2-3 (pC1/1GAPSOD) and AB110 (pC1/1GAPATi9), producing human .alpha..sub.1 -antitrypsin and superoxide dismutase, were desposited at the A.T.C.C. on May 9, 1984 and given Accession Nos. 20708 and 20709, respectively; and 2150-2-3 (GAP5), 2150-2-3 (Pyk5) and 2150-2-3 (PHO5GAP1), expressing Hepatitis B surface antigen, were deposited at the A.T.C.C. on May 9, 1984 and given Accession Nos. 20705, 20706 and 20707, respectively.
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