发明授权
- 专利标题： Chemiluminescence assays using stabilized dioxetane derivatives
- 专利标题（中）： 使用稳定的二氧杂环丁烷衍生物的化学发光测定
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申请号： US382112申请日： 1989-07-19
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公开(公告)号： US5094939A公开(公告)日： 1992-03-10
- 发明人: Masahisa Okada , Yoshihiro Ashihara , Tadashi Ninomiya , Akira Yano
- 申请人： Masahisa Okada , Yoshihiro Ashihara , Tadashi Ninomiya , Akira Yano
- 申请人地址： JPX Tokyo
- 专利权人： Fujirebio, Inc.
- 当前专利权人： Fujirebio, Inc.
- 当前专利权人地址： JPX Tokyo
- 优先权： JPX63-178193 19880719; JPX63-178194 19880719
- 主分类号： C07F9/655
- IPC分类号： C07F9/655 ; C12Q1/42 ; C12Q1/68
摘要：
A multi-pH chemiluminescence-based assay method comprising first-stage light production by the activation by cleavage of a phosphatase cleavable dioxetane derivative within a pH range optimal for enzyme catalysis, followed by the second-stage generation of increased light energy from the products of enzyme-catalyzed cleavage of the dioxetane derivative by the adjustment of the pH to a strongly alkaline pH optimal for the generation of light energy. The assay method is suitable for both solution phase and solid state assay formats. Light generation enhancers can be used further to increase the light yield.
公开/授权文献
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