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    Immunoassay element and process for immunoassay
    2.
    发明授权
    Immunoassay element and process for immunoassay 失效
    免疫测定元件和免疫测定方法

    公开(公告)号：US06589754B1

    公开(公告)日：2003-07-08

    申请号：US08946685

    申请日：1997-10-08

    申请人： Toshikage Hiraoka ,  Tetsuji Tanimoto ,  Yoshihiko Makino ,  Tadashi Ninomiya ,  Hiroshi Shinoki ,  Yoshihiro Ashihara ,  Naofumi Hora ,  Masashi Ogawa

    发明人： Toshikage Hiraoka ,  Tetsuji Tanimoto ,  Yoshihiko Makino ,  Tadashi Ninomiya ,  Hiroshi Shinoki ,  Yoshihiro Ashihara ,  Naofumi Hora ,  Masashi Ogawa

    IPC分类号： G01N3353

    CPC分类号： G01N33/54386

    摘要： An immunoassay element for quantitatively analyzing a macromolecular antigen by determining the change in enzymatic activity caused by a reaction between the macromolecular polymer antigen and an enzyme-labelled antibody. The immunoassay element comprises a substrate layer containing a non-diffusible substrate which forms a diffusible material in the presence of the enzyme, and a reagent layer containing a fragmenting enzyme for further fragmenting the diffusible material into a lower molecular weight product. Also provided is a process for quantitatively analyzing a macromolecular antigen in a sample by the use of the immunoassay element.

    摘要翻译： 一种用于通过测定由高分子聚合物抗原和酶标记的抗体之间的反应引起的酶活性的变化来定量分析大分子抗原的免疫测定元件。 免疫测定元件包括含有在酶存在下形成可扩散材料的不扩散基底的基底层和含有用于进一步将可扩散材料分解成较低分子量产物的碎裂酶的试剂层。 还提供了通过使用免疫测定元件定量分析样品中的大分子抗原的方法。

    Chemiluminescence enhancer
    4.
    发明申请
    Chemiluminescence enhancer 审中-公开

    公开(公告)号：US20070059786A1

    公开(公告)日：2007-03-15

    申请号：US11447839

    申请日：2006-06-07

    申请人： Kazushige Moriyama ,  Tetsuji Tanimoto ,  Tatsuki Matsuno ,  Yoshihiro Ashihara

    发明人： Kazushige Moriyama ,  Tetsuji Tanimoto ,  Tatsuki Matsuno ,  Yoshihiro Ashihara

    IPC分类号： G01N33/551 ,  C07D305/02

    CPC分类号： G01N33/533 ,  G01N21/76 ,  G01N33/532

    摘要： The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.

    Chemiluminescence enhancer
    5.
    发明申请
    Chemiluminescence enhancer 有权
    化学发光增强剂

    公开(公告)号：US20050239149A1

    公开(公告)日：2005-10-27

    申请号：US10518586

    申请日：2003-06-24

    申请人： Kazushige Moriyama ,  Tetsuji Tanimoto ,  Tatsuki Matsuno ,  Yoshihiro Ashihara

    发明人： Kazushige Moriyama ,  Tetsuji Tanimoto ,  Tatsuki Matsuno ,  Yoshihiro Ashihara

    IPC分类号： G01N21/76 ,  G01N33/532 ,  G01N33/533 ,  G01N33/53 ,  G01N33/537 ,  G01N33/543 ,  C07D35/02

    CPC分类号： G01N33/533 ,  G01N21/76 ,  G01N33/532

    摘要： The present invention provides a chemiluminescence enhancer treated to retain favorable dispersibility of fine solid carriers and stably exert a chemiluminescence enhancing action. The invention provides a chemiluminescence enhancer used for signal detection in a solid phase immunoassay using antigen or/and antibody immobilized onto fine solid carriers dispersible in a liquid medium, consisting of a water soluble macromolecular quaternary ammonium salt, a quaternary sulfonium salt or a quaternary phosphonium salt in order to enhance emission of light caused by an enzymatic reaction of a chemiluminescent substrate having dioxetane, wherein the chemiluminescence enhancer is given an aggregation inhibition treatment of the fine solid carriers by the treatment with an oxidizing agent or a reducing agent, and a chemiluminescence method and a kit using the chemiluminescence enhancer.

    摘要翻译： 本发明提供一种化学发光增强剂，其处理以保持细固体载体的良好分散性并稳定地发挥化学发光增强作用。 本发明提供了一种化学发光增强剂，其用于固相免疫测定中的信号检测，所述抗原或/和抗体固定在可分散于液体介质中的细小固体载体上，该水溶液由水溶性大分子季铵盐，季锍盐或季鏻 盐，以增强由具有二氧杂环丁烷的化学发光底物的酶反应引起的光的发射，其中通过用氧化剂或还原剂处理化学发光增强剂对细小固体载体进行聚集抑制处理和化学发光 方法和使用化学发光增强剂的试剂盒。

    Method of measuring biological ligand
    8.
    发明授权
    Method of measuring biological ligand 失效
    生物配体测定方法

    公开(公告)号：US4649105A

    公开(公告)日：1987-03-10

    申请号：US603499

    申请日：1984-04-24

    申请人： Yasushi Kasahara ,  Yoshihiro Ashihara ,  Hiromasa Suzuki

    发明人： Yasushi Kasahara ,  Yoshihiro Ashihara ,  Hiromasa Suzuki

    IPC分类号： G01N33/53 ,  G01N33/542 ,  G01N33/543 ,  G01N33/545 ,  G01N33/58 ,  G01N33/569 ,  G01N33/574

    CPC分类号： G01N33/545 ,  G01N33/54306 ,  G01N33/581 ,  Y10S436/813 ,  Y10S436/817

    摘要： A method of measuring a biological ligand comprises allowing to coexist a biologically active composition comprising an immobilization phase of a particular antibody or a particular ligand and an immobilization phase of a biotinyl enzyme or a biotinyl enzyme inhibitor, a water-soluble conjugate of the ligand or the antibody and the biotinyl enzyme inhibitor or the biotinyl enzyme, and the ligand to be measured in an aqueous solution, and measuring the remaining biotinyl enzyme activity or the biotinyl enzyme inhibitory activity. This method is highly sensitive and specific, and it is suitable as a clinical test for the determination of physiological substances and trace components in humoral fluid.

    摘要翻译： 测量生物配体的方法包括允许共存包含特定抗体或特定配体的固定相的生物活性组合物和生物素酶或生物素酶抑制剂的固定相，配体的水溶性缀合物或 抗体和生物素抑制剂或生物素酶，以及在水溶液中待测量的配体，并测量剩余的生物素酶活性或生物素酶抑制活性。 该方法灵敏度高，具有特异性，适合作为体液中生理物质和微量成分测定的临床试验。

    Nonspecific hybridization inhibitors, clinical examination reagents and clinical examination method
    10.
    发明授权
    Nonspecific hybridization inhibitors, clinical examination reagents and clinical examination method 有权
    非特异性杂交抑制剂，临床检查试剂和临床检查方法

    公开(公告)号：US08252531B2

    公开(公告)日：2012-08-28

    申请号：US10476069

    申请日：2002-04-25

    申请人： Shujiro Sakaki ,  Kenshiro Shuto ,  Mamoru Tsuchida ,  Yoshihiro Ashihara ,  Mitsuo Isomura

    发明人： Shujiro Sakaki ,  Kenshiro Shuto ,  Mamoru Tsuchida ,  Yoshihiro Ashihara ,  Mitsuo Isomura

    IPC分类号： C12Q1/68

    CPC分类号： C12Q1/6832

    摘要： The present invention relates to an inhibitor capable of increasing accuracy of the reaction in hybridization, and inhibiting nonspecific hybridization, a clinical diagnostic reagent, and a method of clinical analysis whereby nonspecific hybridization in clinical analysis is inhibited, and a target nucleic substance is detected easily with high accuracy. The inhibitor and the clinical diagnostic reagent contains polymer (H) having a nonspecific hybridization inhibitory action. The polymer has in its molecule at least one of carboxyl and sulfone groups, and phosphorylcholine-like groups, and has a weight average molecular weight of 1000 to 5000000. The method of clinical analysis includes contacting a sample with a test agent capable of hybridizing with a specific nucleic substance in the presence of the inhibitor.

    摘要翻译： 本发明涉及能够提高杂交反应精度，抑制非特异性杂交的抑制剂，临床诊断试剂和临床分析方法，临床分析中的非特异性杂交被抑制，容易检测到靶核酸 准确度高。 抑制剂和临床诊断试剂含有具有非特异性杂交抑制作用的聚合物（H）。 聚合物在其分子中具有羧基和砜基中的至少一个和磷酸胆碱样基团，并且具有1000至5000000的重均分子量。临床分析方法包括使样品与能够与 在抑制剂存在下的特定核酸物质。