Five subdomains of the CaMV35S promoter are provided that cause tissue specific and/or developmentally regulated expression of chimeric genes in plants. These subdomains act as promoters for use in transformed plant cells, seeds and transgenic plants. Some of the subdomains require fusion to domain A for expression. Subdomains B2, B3, B4, and B5 exhibit expression when fused to the minimal promoter sequence in mature plants whereas only B2 and B3 confer expression in seeds and only B2, B3 and B4 confer expression at the seedling stage of development. The combination of subdomains B4 and B5 confers expression at all stages of development as does the B1+TGACG motif combination when each combination is fused with the minimal promoter sequence. The nucleotide sequence and DNA molecule that function as the enhancers are provided.