发明授权
US5176996A Method for making synthetic oligonucleotides which bind specifically to
target sites on duplex DNA molecules, by forming a colinear triplex,
the synthetic oligonucleotides and methods of use
失效
通过形成共线三联制备合成寡核苷酸的方法，其特异性结合双链DNA分子上的靶位点，合成寡核苷酸和使用方法
- 专利标题： Method for making synthetic oligonucleotides which bind specifically to target sites on duplex DNA molecules, by forming a colinear triplex, the synthetic oligonucleotides and methods of use
- 专利标题（中）： 通过形成共线三联制备合成寡核苷酸的方法，其特异性结合双链DNA分子上的靶位点，合成寡核苷酸和使用方法
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申请号： US453532申请日： 1989-12-22
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公开(公告)号： US5176996A公开(公告)日： 1993-01-05
- 发明人: Michael E. Hogan , Donald J. Kessler
- 申请人： Michael E. Hogan , Donald J. Kessler
- 申请人地址： TX Houston
- 专利权人： Baylor College of Medicine
- 当前专利权人： Baylor College of Medicine
- 当前专利权人地址： TX Houston
- 主分类号： A61K38/00
- IPC分类号： A61K38/00 ; A61K48/00 ; C07H21/00 ; C07H21/04 ; C12N15/113 ; C12Q1/68 ; C12Q1/70
摘要：
A method for making synthetic oligonucleotides which bind to target sequences in a duplex DNA forming colinear triplexes by binding to the major groove. The method includes scanning genomic duplex DNA and identifying nucleotide target sequences of greater than about 20 nucleotides having either about at least 65% purine bases or about at least 65% pyrimidine bases; and synthesizing synthetic oligonucleotides complementary to identified target sequences. The synthetic oligonucleotides have a G when the complementary location in the DNA duplex has a GC base pair and have a T when the complementary location in the DNA duplex has an AT base pair. The synthetic oligonucleotides are oriented 5' to 4' and bind parallel or 3' to 5' and bind anti-parallel to the about at least 65% purine strand.
公开/授权文献
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