A heat transfer sheet including a substrate film and a dye layer including a dye and a binder formed thereon, wherein the binder includes organic-solvent-soluble polymers having ester moieties and/or urethane moieties, prepared by modifying water-soluble natural and/or semisynthetic polymers by esterifying and/or urethanating an inner hydroxyl group thereof, and the dye includes a sublimable dye homogeneously dissolved in the binder. A heat transfer sheet is also disclosed which includes a substrate film, and a dye layer formed thereon, wherein the dye layer includes (i) a binder which is cellulose acetate having an acetylation rate of 2.4 or more and a total substitution rate of 2.7 or more, and (ii) a sublimable dye dissolved in the binder. The sublimable dye contained in the dye layer is not separated from the binder even when the heat transfer sheet is preserved for a long period of time. Furthermore, the heat transfer sheet can produce a high-density image even when only a small amount of thermal energy is applied thereto.