发明授权
- 专利标题: Host transformed with yeast gene and ubiquitin/polypeptide fusions
- 专利标题(中): 宿主用酵母基因和泛素/多肽融合物转化
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申请号: US694522申请日: 1991-04-30
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公开(公告)号: US5262322A公开(公告)日: 1993-11-16
- 发明人: Chung-Cheng Liu , Harvey I. Miller
- 申请人: Chung-Cheng Liu , Harvey I. Miller
- 申请人地址: CA South San Francisco
- 专利权人: Genentech, Inc.
- 当前专利权人: Genentech, Inc.
- 当前专利权人地址: CA South San Francisco
- 主分类号: C07K14/00
- IPC分类号: C07K14/00 ; C07K14/64 ; C12N9/60 ; C12N15/57 ; C12N1/21
摘要:
A ubiquitin hydrolase is provided having a purity of at least 70% homogeneity based on the weight of the total protein in the composition. Also provided are DNA sequences encoding ubiquitin hydrolases, as well as expression systems for their recombinant production. Processes are provided for purification of a ubiquitin hydrolase from eukaryotes and for its use in recovering any desired polypeptide free from its fusion at its N-terminus with ubiquitin.
公开/授权文献
- US5771097A Mach-zehnder type interferometer 公开/授权日:1998-06-23
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