发明授权
- 专利标题： Cephalosporin C acylase
- 专利标题（中）： 头孢菌素C酰基转移酶
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申请号： US935312申请日： 1992-08-26
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公开(公告)号： US5320948A公开(公告)日： 1994-06-14
- 发明人: Morita Iwami , Ichiro Aramori , Masao Fukagawa , Takao Isogai , Hitoshi Kojo
- 申请人： Morita Iwami , Ichiro Aramori , Masao Fukagawa , Takao Isogai , Hitoshi Kojo
- 申请人地址： JPX Osaka
- 专利权人： Fujisawa Pharmaceutical Co., Ltd.
- 当前专利权人： Fujisawa Pharmaceutical Co., Ltd.
- 当前专利权人地址： JPX Osaka
- 优先权： GBX9019724 19900910
- 主分类号： C12N1/21
- IPC分类号： C12N1/21 ; C12N9/80 ; C12N15/09 ; C12N15/56 ; C12P35/04 ; C12P35/06 ; C12R1/19 ; C12R1/38 ; C12P35/00 ; C12P21/02
摘要：
A cephalosporin C acylase from Pseudomonas diminuta N-176 is characterized by its ability to catalyze the conversion of cephalosporin C, glutaryl 7-ACA, adipyl 7-ACA, succinyl 7-ACA, N-acetylcephalosporin, N-benzoylcephalosporin C and cephalothin into 7-aminocephalosporanic acid. The enzyme contains an .alpha.-subunit having a molecular weight of 26 kDA and a .beta.-subunit having a molecular weight of 58 kDa. A method for the recombinant production of the present cephalosporin C acylase in Escherichia coli is provided.
公开/授权文献
- US4814083A Altering pore size distributions 公开/授权日：1989-03-21