发明授权
- 专利标题: Indirect fluorescent assay of blood samples
- 专利标题(中): 血液样品的间接荧光检测
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申请号: US192629申请日: 1994-02-07
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公开(公告)号: US5460979A公开(公告)日: 1995-10-24
- 发明人: Robert A. Levine , Stephen C. Wardlaw , Leon W. M. M. Terstappen , Thomas J. Mercolino , Diether J. Recktenwald
- 申请人: Robert A. Levine , Stephen C. Wardlaw , Leon W. M. M. Terstappen , Thomas J. Mercolino , Diether J. Recktenwald
- 申请人地址: NJ Franklin Lakes
- 专利权人: Becton Dickinson and Company
- 当前专利权人: Becton Dickinson and Company
- 当前专利权人地址: NJ Franklin Lakes
- 主分类号: G01N33/543
- IPC分类号: G01N33/543 ; B01L3/14 ; G01N33/49 ; G01N33/537 ; G01N33/569 ; G01N33/58 ; G01N33/538 ; G01N33/546
摘要:
A patient's health is diagnosed by centrifuging blood samples in a transparent tube, which tube contains one or more groups of particles such as lyposomes or plastic beads of different densities for each group. Each group of density-defined particles carries antigens or antibodies which are specific to a complement antigen or antibody which may be in the blood sample being tested, and which are indicative of the patient's health. A label-tagged antibody which is specific to all bound antibody/antigen couples is added to the blood sample so as to form labelled antibody+antigen-antibody complexes (AAAC) in the blood sample. Upon centrifugation, the complexed particles will settle out in different areas in the tube according to the respective density of the particles, and the degree of label emission of the particle layers can enable qualitative or quantitative analyses of the blood sample to be made. Unbound labelled antibodies will be washed away from the complexed layers by the washing action of the descending blood cells during the centrifugation step. Unbound labelled antibodies will thus not interfere with the analysis.
公开/授权文献
- US4816544A Polyamideimide elastomer and production thereof 公开/授权日:1989-03-28
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