发明授权
US5482847A Esterase genes, esterase, recombinant plasmids and transformants containing the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters using said trasnformants 失效
酯酶基因,酯酶,重组质粒和含有重组质粒的转化体和使用所述trasnformants制备光学活性羧酸及其对映体酯的方法

Esterase genes, esterase, recombinant plasmids and transformants
containing the recombinant plasmid and methods of producing optically
active carboxylic acids and their enantiomeric esters using said
trasnformants
摘要:
The present invention relates to a DNA fragment containing a nucleotide sequence that encodes an amino acid sequence of esterase, said esterase asymmetrically hydrolyzing carboxylic acid esters represented by the formula (I); ##STR1## (wherein R.sub.1 is alkyl, aralkyl or aryl, R.sub.2 and R.sub.3 are alkyl, and n is 1 or 2) an esterase encoded by the DNA fragment, a recombinant plasmid containing the DNA fragment, a microorganism transformed with the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters.
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