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1.发明授权
公开(公告)号：US5773240A
公开(公告)日：1998-06-30
申请号：US750761
申请日：1996-12-12
申请人： Eiji Ozaki , Toshitaka Uragaki , Keiichi Sakashita , Tetsuya Ikemoto , Yoshimasa Kobayashi , Akihiro Sakimae
发明人： Eiji Ozaki , Toshitaka Uragaki , Keiichi Sakashita , Tetsuya Ikemoto , Yoshimasa Kobayashi , Akihiro Sakimae
IPC分类号： C07C53/128 , C07C55/02 , C07C55/32 , C07C57/13 , C07C59/01 , C07C59/115 , C07C69/34 , C07C69/593 , C07C69/675 , C12P41/00 , C12P1/00 , C12P7/44
CPC分类号： C07C69/593 , C07C53/128 , C07C55/02 , C07C55/32 , C07C57/13 , C07C59/01 , C07C59/115 , C07C69/34 , C07C69/675 , C12P41/004
摘要： The present invention relates to an optically active .alpha.-substituted carboxylic acid derivative represented by the general formula (I): ##STR1## (wherein R.sub.1 is a hydroxyl group, ##STR2## R.sub.2 is a methyl group or a chlorine atom; R.sub.3 is a hydrogen atom or an alkyl group with 1-6 carbon atoms; n is an integer of 1 or 2; and * represents an asymmetric carbon atom, provided that R.sub.2 is a chlorine atom when R.sub.1 is a hydroxyl group); and a method for producing the optically active .alpha.-substituted carboxylic acid derivative represented by the general formula (I) and antipodes thereof using a culture, cells or a material obtainable from cells of a microorganism having an ability to asymmetrically hydrolyze ester bonds. The optically active .alpha.-substituted carboxylic acid derivatives and antipodes thereof provided by the invention are useful as raw materials for various liquid crystals and as synthetic intermediates for various optically active medicines or agricultural chemicals.
摘要翻译： PCT No.PCT / JP95 / 01176 Sec。 371日期：1996年12月12日 102（e）日期1996年12月12日PCT提交1995年6月13日PCT公布。 第WO95 / 34525号公报 日期：1995年12月21日本发明涉及由通式（I）表示的光学活性α-取代羧酸衍生物：其中R1是羟基，R2是甲基 或氯原子; R3是氢原子或具有1-6个碳原子的烷基; n是1或2的整数; *表示不对称碳原子，条件是当R1是羟基时R2是氯原子 组）; 以及使用可以从具有不对称水解酯键的能力的微生物的细胞获得的培养物，细胞或材料制备由通式（I）表示的光学活性α-取代羧酸衍生物及其对映体的方法。 本发明提供的光学活性的α-取代羧酸衍生物及其对映体可用作各种液晶的原料，也可用作各种光学活性药物或农用化学品的合成中间体。
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2.发明授权Process for the preparation of organic esters of ascorbic acid or erythorbic acid using ester hydralases in organic solvents containing 100-10,000 ppm of water 失效在含有100-10,000 PPM水的有机溶剂中使用酯类水合物制备抗坏血酸或硫酸的有机酸的方法
公开(公告)号：US5079153A
公开(公告)日：1992-01-07
申请号：US531701
申请日：1990-06-01
IPC分类号： C12P17/04
CPC分类号： C12P17/04
摘要： A process for the preparation of an organic ester of ascorbic acid or erythorbic acid represented by the general formula (II) or (III): ##STR1## wherein R.sub.1 is alkyl, aralkyl, or aryl, by reacting ascorbic acid or erythorbic acid with an organic acid or an ester thereof of the general formula (I) :R.sub.1 COOR.sub.2 (I)wherein R.sub.1 is as defined above and R.sub.2 is hydrogen, methyl, ethyl or propyl, in an organic solvent in the presence of an ester hydrolase.
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公开(公告)号：US5597716A
公开(公告)日：1997-01-28
申请号：US340996
申请日：1994-11-17
申请人： Eiji Sato , Eiji Ozaki , Chinami Iida , Yoshimasa Kobayashi , Akihiro Sakimae
发明人： Eiji Sato , Eiji Ozaki , Chinami Iida , Yoshimasa Kobayashi , Akihiro Sakimae
CPC分类号： C12P7/56 , C12P41/006 , Y10S435/822 , Y10S435/829 , Y10S435/84 , Y10S435/843 , Y10S435/874 , Y10S435/876 , Y10S435/877
摘要： The present invention relates to a process for producing D-lactic acid and L-lactamide, comprising allowing a culture broth of a microorganism capable of asymmetric hydrolysis of DL-lactamide belonging to the genus Alcaligenes, Pseudomonas, Agrobacterium, Brevibacterium, Acinetobacter, Corynebacterium, Enterobacter, Micrococcus or Rhodococcus, the microorganism itself, a material obtained therefrom or an immobilized material thereof to act on DL-lactamide, and recovering the resulting D-lactic acid and the remaining L-lactamide. The present invention enables sufficient production of D-lactic acid and L-lactamide by the present microorganism.
摘要翻译： 本发明涉及D-乳酸和L-乳酰胺的制造方法，其特征在于，含有能够产生属于产碱杆菌属，假单胞菌属，农杆菌属，短杆菌属，不动杆菌属，棒状杆菌属， 肠杆菌属，微球菌属或红球菌属，微生物本身，由其获得的材料或其固定化材料作用于DL-乳酰胺，并回收得到的D-乳酸和剩余的L-乳酰胺。 本发明能够通过本发明的微生物充分生产D-乳酸和L-乳酰胺。
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4.发明授权Process for the stereoselective esterification of ascorbic or erythorbic acids with long-chained enol esters 失效用长链烯醇酯立体选择性地酯化抗坏血酸或异抗坏血酸的方法
公开(公告)号：US5455174A
公开(公告)日：1995-10-03
申请号：US117360
申请日：1993-09-07
CPC分类号： C12P17/04
摘要： An organic acid ester of ascorbic acid or erythorbic acid is produced by reacting ascorbic acid or erythorbic acid with an organic acid enol ester in an organic solvent with a solubility of ascorbic acid or erythorbic acid of more than 0.3% at 25.degree. C. in the presence of an active lipase.
摘要翻译： 抗坏血酸或异抗坏血酸的有机酸酯通过在有机溶剂中使抗坏血酸或异抗坏血酸与有机酸烯醇酯反应，在25℃下使抗坏血酸或异抗坏血酸的溶解度大于0.3％ 活性脂肪酶的存在。
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5.发明授权
公开(公告)号：US5071753A
公开(公告)日：1991-12-10
申请号：US607676
申请日：1990-11-01
IPC分类号： C12P17/04
CPC分类号： C12P17/04
摘要： A process for the preparation of an organic ester of ascorbic acid or erythorbic acid represented by the general formula (II) or (III): ##STR1## wherein R.sub.1 is alkyl, aralkyl, or aryl, by reacting ascorbic acid or erythorbic acid with an organic acid or an ester thereof of the general formula (I):wherein R.sub.1 is as defined above and R.sub.2 is hydrogen, methyl, ethyl or propyl, in an organic solvent in the presence of an ester hydrolase.
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6.发明授权Esterase genes, esterase, recombinant plasmids and transformants containing the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters using said trasnformants 失效酯酶基因，酯酶，重组质粒和含有重组质粒的转化体和使用所述trasnformants制备光学活性羧酸及其对映体酯的方法
公开(公告)号：US5482847A
公开(公告)日：1996-01-09
申请号：US183213
申请日：1994-01-14
申请人： Eiji Ozaki , Akihiro Sakimae , Ryozo Numazawa
发明人： Eiji Ozaki , Akihiro Sakimae , Ryozo Numazawa
CPC分类号： C12P11/00 , C12N9/18 , C12P41/005 , C12P7/40
摘要： The present invention relates to a DNA fragment containing a nucleotide sequence that encodes an amino acid sequence of esterase, said esterase asymmetrically hydrolyzing carboxylic acid esters represented by the formula (I); ##STR1## (wherein R.sub.1 is alkyl, aralkyl or aryl, R.sub.2 and R.sub.3 are alkyl, and n is 1 or 2) an esterase encoded by the DNA fragment, a recombinant plasmid containing the DNA fragment, a microorganism transformed with the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters.
摘要翻译： 本发明涉及包含编码酯酶氨基酸序列的核苷酸序列的DNA片段，所述酯酶不对称水解的由式（I）表示的羧酸酯; （其中R1是烷基，芳烷基或芳基，R2和R3是烷基，n是1或2）由DNA片段编码的酯酶，含有该DNA片段的重组质粒，用重组质粒转化的微生物和 制备光学活性羧酸及其对映体酯的方法。
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7.发明授权
公开(公告)号：US5308765A
公开(公告)日：1994-05-03
申请号：US882329
申请日：1992-05-13
申请人： Eiji Ozaki , Akihiro Sakimae , Ryozo Numazawa
发明人： Eiji Ozaki , Akihiro Sakimae , Ryozo Numazawa
CPC分类号： C12P11/00 , C12N9/18 , C12P41/005 , C12P7/40
摘要： The present invention relates to a DNA fragment containing a nucleotide sequence that encodes an amino acid sequence of esterase, said esterase asymmetrically hydrolyzing carboxylic acid esters represented by the formula (I); ##STR1## (wherein R.sub.1 is alkyl, aralkyl or aryl, R.sub.2 and R.sub.3 are alkyl, and n is 1 or 2) an esterase encoded by the DNA fragment, a recombinant plasmid containing the DNA fragment, a microorganism transformed with the recombinant plasmid and methods of producing optically active carboxylic acids and their enantiomeric esters.
摘要翻译： 本发明涉及包含编码酯酶氨基酸序列的核苷酸序列的DNA片段，所述酯酶不对称水解的由式（I）表示的羧酸酯; （其中R1是烷基，芳烷基或芳基，R2和R3是烷基，n是1或2）由DNA片段编码的酯酶，含有该DNA片段的重组质粒，用重组质粒转化的微生物和 制备光学活性羧酸及其对映体酯的方法。
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公开(公告)号：US5149855A
公开(公告)日：1992-09-22
申请号：US627779
申请日：1990-12-14
IPC分类号： C07C327/32
CPC分类号： C07C327/32
摘要： Process for racemizing an optically active carboxylic acid ester of the formula (1): ##STR1## wherein R.sub.1 is alkyl, aralkyl or aryl, R.sub.2 and R.sub.3 independently are alkyl, and n is 1 or 2, which comprises contacting the compound of the formula (1) with an amine compound.
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公开(公告)号：US5149649A
公开(公告)日：1992-09-22
申请号：US561323
申请日：1990-08-01
摘要： A method for culturing cells by supplying oxygen to a culture medium through a multilayer composite membrane consisting of a porous layer(s) and a nonporous layer(s) having a thickness of less than 10 micrometers laminated one after the other. By using this multilayer composite membrane for supplying oxygen to the culture medium, oxygen can be supplied to the culture medium and/or the culture broth without causing foaming in the culture medium, cell growth inhibition or reduction in productivity of cellular products.
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10.发明授权Process for preparing optically active carboxylic acids and antipode esters thereof 失效光学活性羧酸及其对映体酯的制备方法
公开(公告)号：US4629701A
公开(公告)日：1986-12-16
申请号：US627093
申请日：1984-07-02
申请人： Akihiro Sakimae , Yuri Kagawa , Ryozo Numazawa , Hisao Onishi
发明人： Akihiro Sakimae , Yuri Kagawa , Ryozo Numazawa , Hisao Onishi
摘要： Organic carboxylic acid ester represented by the formula (II): ##STR1## wherein R.sub.1 is an alkyl, an aralkyl or an aryl group; R.sub.2 is an alkyl group; R.sub.3 is an alkyl group; n is 1 or 2, are synthesized in racemic form, and then the acid ester (II) is treated with a source containing enzyme capable of rearranging with or without asymmetrically hydrolyzing ester bond until either a d-form or 1-form optically active carboxylic acid ester represented by the formula (II) with or without the antipode acid thereof is produced.
摘要翻译： 由式（II）表示的有机羧酸酯：其中R 1是烷基，芳烷基或芳基; R2是烷基; R3是烷基; n为1或2，以外消旋形式合成，然后用含有酶的酶处理酸酯（II），其能够或不具有不对称水解的酯键重排，直到d型或1-型光学活性羧基 制备了具有或不具有对映体酸的由式（II）表示的酸酯。
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