An artificial insemination and embryo transfer device comprises an elongate hollow tube, and a hollow straw containing a reproductive organism therein and fitted in the elongate tube. A flexible tip is secured to an end of the elongate tube and engaged with the straw. The tip has a passage in communication with the straw and a conical portion adapted to smoothly pass it through a cervical canal of a female subject. A piston rod is slidably mounted in the elongate tube and constructed to enter into the straw so as to discharge the reproductive organism contained in the straw through the passage of the tip by its axial advancement. A locking element is disposed between the elongate tube and the piston rod for restraining a free axial movement of the piston rod within the elongate tube. A first enclosure is inserted around the elongate tube so as to protect the elongate tube against contamination from infectious materials in the cervical canal during the passing of the device through the cervical canal. A second enclosure surrounds the tip and the first enclosure for preventing them from being contaminated from infectious materials in a vaginal canal during the insertion of the device into the vaginal canal.