发明授权
- 专利标题: Polymerase chain reaction container and methods of using the same
- 专利标题(中): 聚合酶链反应容器及其使用方法
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申请号: US418537申请日: 1995-04-07
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公开(公告)号: US5576197A公开(公告)日: 1996-11-19
- 发明人: Robert Arnold
- 申请人: Robert Arnold
- 申请人地址: CA San Diego
- 专利权人: Molecular Bio-Products
- 当前专利权人: Molecular Bio-Products
- 当前专利权人地址: CA San Diego
- 主分类号: B01L3/14
- IPC分类号: B01L3/14 ; C12Q1/68 ; G01N35/00 ; C12P19/34 ; C10G73/36
摘要:
The invention describes a polymerase chain reaction container and methods of using the same that greatly simplify and improve the polymerase chain reaction. Specifically, the invention describes a PCR container comprising a container suitable for PCR with wax attached to the inside surface of the container. The wax is positioned at or above an estimated meniscus position of a PCR mixture. The wax melts at PCR temperatures and covers the surface of the PCR mixture thereby prevents evaporation during thermal cycling. The invention is superior to current methods, in part, because it reduces preparation time, potential sources of contamination and adverse effects on PCR.
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