发明授权
US5589362A Tetracycline regulated transcriptional modulators with altered DNA
binding specificities
失效
具有改变的DNA结合特异性的四环素调节的转录调节子
- 专利标题: Tetracycline regulated transcriptional modulators with altered DNA binding specificities
- 专利标题(中): 具有改变的DNA结合特异性的四环素调节的转录调节子
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申请号: US485971申请日: 1995-06-07
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公开(公告)号: US5589362A公开(公告)日: 1996-12-31
- 发明人: Hermann Bujard , Manfred Gossen , Wolfgang Hillen , Vera Helbl , Dirk Schnappinger
- 申请人: Hermann Bujard , Manfred Gossen , Wolfgang Hillen , Vera Helbl , Dirk Schnappinger
- 申请人地址: DEX Ludwigshafen DEX Ludwigshafen
- 专利权人: BASF Aktiengesellschaft,Knoll Aktiengesellschaft
- 当前专利权人: BASF Aktiengesellschaft,Knoll Aktiengesellschaft
- 当前专利权人地址: DEX Ludwigshafen DEX Ludwigshafen
- 主分类号: A01K67/027
- IPC分类号: A01K67/027 ; A61K48/00 ; C07K14/00 ; C07K14/035 ; C07K14/195 ; C07K14/245 ; C07K19/00 ; C12N15/11 ; C12N15/63 ; C12N15/67 ; C12N15/85 ; C12P21/00 ; C07H21/04 ; C12N15/31
摘要:
Isolated nucleic acid molecules encoding fusion proteins which regulate transcription in eukaryotic cells are disclosed. The fusion proteins of the invention comprise a Tet repressor having at least one amino acid mutation that confers on the fusion protein an ability to bind a class B tet operator sequence having a nucleotide substitution at position +4 or +6, operatively linked to a polypeptide which regulates transcription in eukaryotic cells. Methods for regulating transcription of a tet operator-linked gene in a cell are also provided. In one embodiment, the method involves introducing into the cell a nucleic acid molecule encoding a fusion protein which regulates transcription, the fusion protein comprising a Tet repressor having at least one amino acid mutation that confers on the fusion protein an ability to bind a class B tet operator sequence having a nucleotide substitution at position +4 or +6, operatively linked to a polypeptide which regulates transcription in eukaryotic cells, and modulating the concentration of a tetracycline, or analogue thereof, in contact with the cell.
公开/授权文献
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