发明授权
- 专利标题: Control of norleucine incorporation into recombinant proteins
- 专利标题(中): 正亮氨酸掺入重组蛋白的控制
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申请号: US363862申请日: 1994-12-22
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公开(公告)号: US5599690A公开(公告)日: 1997-02-04
- 发明人: Dennis Fenton , Por-Hsiung Lai , Hsieng Lu , Michael Mann , Larry Tsai
- 申请人: Dennis Fenton , Por-Hsiung Lai , Hsieng Lu , Michael Mann , Larry Tsai
- 申请人地址: CA Thousand Oaks
- 专利权人: Amgen Inc.
- 当前专利权人: Amgen Inc.
- 当前专利权人地址: CA Thousand Oaks
- 主分类号: C07K14/52
- IPC分类号: C07K14/52 ; C12P21/02 ; C12P21/04
摘要:
Norleucine incorporation into recombinant gene products may be inhibited by culturing the cells expressing the gene products in media comprising a high level of leucine and/or methionine, or by expressing the gene products in a leu mutant. Norleucine incorporation in recombinant gene products may be promoted by culturing the cells expressing the gene products in media comprising norleucine or deficient in leucine and/or methionine. For example, norleucine analogs of IL-2, GCSF, .gamma.-interferon and .alpha.-consensus interferon may be produced in this way.
公开/授权文献
- US5090797A Method and apparatus for mirror control 公开/授权日:1992-02-25
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