发明授权
- 专利标题: Method for immobilizing nucleic acid molecules
- 专利标题(中): 固定核酸分子的方法
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申请号: US341148申请日: 1994-11-16
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公开(公告)号: US5610287A公开(公告)日: 1997-03-11
- 发明人: Theo Nikiforov , Michael R. Knapp
- 申请人: Theo Nikiforov , Michael R. Knapp
- 申请人地址: MD Baltimore
- 专利权人: Molecular Tool, Inc.
- 当前专利权人: Molecular Tool, Inc.
- 当前专利权人地址: MD Baltimore
- 主分类号: C12N15/10
- IPC分类号: C12N15/10 ; C12Q1/68 ; C07H21/04 ; C12N11/08 ; C12N15/00
摘要:
Synthetic nucleic acid molecules are non-covalently immobilized in the presence of a salt or cationic detergent on a hydrophilic polystyrene solid support containing an --OH, --C.dbd.O or --COOH hydrophilic group or on a glass solid support. The support is contacted with a solution having a pH of about 6 to about 8 containing the synthetic nucleic acid and the cationic detergent or salt. Preferably, the cationic detergent is 1-ethyl-3-(3'-dimethylaminopropyl)-1,3-carbodiimide hyrochloride at a concentration of about 30 mM to about 100 mM or octyldimethylamine hydrochloride at a concentration of about 50 mM to about 150 mM. The salt is preferably NaCl at a concentration of about 50 mM to about 250 mM. When the detergent is 1-ethyl-3-(3'-dimethylaminopropyl)-1,3-carbodiimide hyrochloride, the glass support or the hydrophilic polystyrene support is used. When NaCl or octyldimethylamine hydrochloride is used, the support is the hydrophilic polystyrene. After immobilization, the support containing the immobilized nucleic acid may be washed with an aqueous solution containing a non-ionic detergent. The immobilized nucleic acid may be used in nucleic acid hybridization assays, nucleic acid sequencing and in analysis of genomic polymorphisms.
公开/授权文献
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