发明授权
US5631144A Application of novel DNA fragments as a coding sequence for a signal
peptide for the secretion of mature proteins by recombinant yeast,
expression cassettes, transformed yeast and corresponding process for
the preparation of proteins
失效
应用新型DNA片段作为信号肽的编码序列，用于通过重组酵母，表达盒，转化酵母和相应的蛋白质制备方法分泌成熟蛋白
- 专利标题： Application of novel DNA fragments as a coding sequence for a signal peptide for the secretion of mature proteins by recombinant yeast, expression cassettes, transformed yeast and corresponding process for the preparation of proteins
- 专利标题（中）： 应用新型DNA片段作为信号肽的编码序列，用于通过重组酵母，表达盒，转化酵母和相应的蛋白质制备方法分泌成熟蛋白
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申请号： US385375申请日： 1995-02-07
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公开(公告)号： US5631144A公开(公告)日： 1997-05-20
- 发明人: Yves Lemoine , Martine Nguyen , Tilman Achstetter , Jean-Marc Reichhart
- 申请人： Yves Lemoine , Martine Nguyen , Tilman Achstetter , Jean-Marc Reichhart
- 申请人地址： FRX Courbevoie
- 专利权人： Transgene S.A.
- 当前专利权人： Transgene S.A.
- 当前专利权人地址： FRX Courbevoie
- 优先权： FRX8905687 19890428; WOXPCT/FR90/00306 19900427
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C07K14/435 ; C07K14/815 ; C12N1/19 ; C12N9/24 ; C12N15/15 ; C12N15/62 ; C12N15/81 ; C12P21/00 ; C12R1/865 ; C12N5/10 ; C12N15/63
摘要：
The invention relates to new DNA fragments and to their application as a DNA coding fragment for a signal peptide which can be used for the secretion of proteins, said peptide including a sequence of amino-acids which show a degree of correspondence of at least 60% with the sequence of amino-acids (I) or (II), preferably with the sequence (II). The sequences (I) and (II) are as follows: (I) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gln. (II) Arg-Phe-Ser-Thr-Thr-Leu-Ala-Thr-Ala-Ala-Thr-Ala-Leu-Phe-Phe-Thr-Ala-Ser-Gln-Val-Ser-Ala.