发明授权
- 专利标题： Method for synthesizing single-stranded stem-loop DNAs, the products and uses therefor
- 专利标题（中）： 单链茎环DNA的合成方法，产品及用途
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申请号： US284860申请日： 1994-08-02
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公开(公告)号： US5643762A公开(公告)日： 1997-07-01
- 发明人: Atsushi Ohshima , Sumiko Inouye , Masayori Inouye
- 申请人： Atsushi Ohshima , Sumiko Inouye , Masayori Inouye
- 申请人地址： NJ Newark
- 专利权人： University of Medicine And Denistry Of New Jersey
- 当前专利权人： University of Medicine And Denistry Of New Jersey
- 当前专利权人地址： NJ Newark
- 主分类号： C12N15/09
- IPC分类号： C12N15/09 ; C12N9/38 ; C12N15/10 ; C12N15/70 ; C12Q1/68 ; C12P19/34 ; C12N15/11
摘要：
The invention provides a method for synthesizing new and useful single-stranded DNAs which have a stem-loop configuration (ss-slDNA). The method is an in vivo or in vitro synthesis. Replicating vehicles are provided which produce these ss-slDNAs. The slDNAs can be used for introducing random mutations in a selected gene, and they lend themselves for replication by a variant of the PCR method.
公开/授权文献
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