发明授权
US5663242A End-attachment of oligonucleotides to polyacrylamide solid supports for
capture and detection of nucleic acids
失效
将寡核苷酸末端连接到用于捕获和检测核酸的聚丙烯酰胺固体支持物上
- 专利标题： End-attachment of oligonucleotides to polyacrylamide solid supports for capture and detection of nucleic acids
- 专利标题（中）： 将寡核苷酸末端连接到用于捕获和检测核酸的聚丙烯酰胺固体支持物上
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申请号： US414019申请日： 1995-03-31
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公开(公告)号： US5663242A公开(公告)日： 1997-09-02
- 发明人: Soumitra Shanker Ghosh , Eoin D. Fahy
- 申请人： Soumitra Shanker Ghosh , Eoin D. Fahy
- 申请人地址： CA La Jolla
- 专利权人： Siska Diagnostics, Inc.
- 当前专利权人： Siska Diagnostics, Inc.
- 当前专利权人地址： CA La Jolla
- 主分类号： C07H21/00
- IPC分类号： C07H21/00 ; C12Q1/68 ; C08F20/56 ; C12P19/34 ; C12Q1/70
摘要：
Methods for covalent attachment of oligonucleotides to solid supports such that substantially all of the oligonucleotides are attached via their 5'-ends are provided. The solid supports with attached oligonucleotides are produced. Thiol-oligonucleotides are attached to bromoacetyl-derivatized polyacrylamide supports, or conversely, bromoacetyl-oligonucleotides are immobilized on thiol-polyacrylamide supports. In a further aspect, bromoacetyl-derivatized oligonucleotides, and polyacrylamide supports with linked oligonucleotides produced by coupling bromoacetyl-derivatized oligonucleotides with thiol-derivatized polyacrylamide solid supports or by coupling thiol-derivatized oligonucleotides with bromoacetyl-derivatized polyacrylamide supports as well as methods for capture of nucleic acids by oligonucleotides attached to polyacrylamide solid supports, either by direct capture or in sandwich hybridization formats are provided.
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