发明授权
US5691138A Nucleotide sequences which hybridize specifically with a Campylobacter
jejuni genomic nucleic sequence
失效
与空肠弯曲杆菌基因组核酸序列特异性杂交的核苷酸序列
- 专利标题: Nucleotide sequences which hybridize specifically with a Campylobacter jejuni genomic nucleic sequence
- 专利标题(中): 与空肠弯曲杆菌基因组核酸序列特异性杂交的核苷酸序列
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申请号: US307591申请日: 1994-09-29
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公开(公告)号: US5691138A公开(公告)日: 1997-11-25
- 发明人: Jean-luc Guesdon , Veronique Stonnet
- 申请人: Jean-luc Guesdon , Veronique Stonnet
- 申请人地址: FRX Paris Cedex
- 专利权人: Institut Pasteur
- 当前专利权人: Institut Pasteur
- 当前专利权人地址: FRX Paris Cedex
- 优先权: FRX9300978 19930129
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; C07H21/04 ; C12Q1/68 ; C07H21/02
摘要:
The subject of the present invention is thus a nucleotide sequence which hybridizes specifically with a Campylobacter jejuni genomic nucleic acid sequence chosen from the nucleotide sequence SEQ ID No. 1, the nucleotide sequence SEQ ID No. 2 the sequences complementary to the latter, as well as the sequences differing therefrom by nutation, insertion, deletion or substitution of one or more bases, and which does not or virtually does not hybridize with nucleic acids from other Campylobacter species. Fragments of this sequence can be used as specific primers for the amplification of sequences specific for Campylobacter jejuni and as nucleic probes specific for Campylobacter jejuni nucleic sequences. The subject of the invention is also a method of detecting the presence of Campylobacter jejuni stains in a biological sample, as well a kit for carrying out the method.
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