A peristaltic pump tube holder includes a body that supports a flexible tubing loop in an erect, outwardly bowed position extending from the body for engagement with a peristaltic pump rotor. The holder also includes a receptacle carrying the body. The receptacle includes a wall forming a chamber that covers the flexible tubing loop and at least partially shields it from contact. The chamber further serves, during engagement between the tubing loop and a peristaltic pump rotor, as a cover for the peristaltic pump rotor.