发明授权
- 专利标题： Gene amplification methods
- 专利标题（中）： 基因扩增方法
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申请号： US644664申请日： 1996-05-01
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公开(公告)号： US5776746A公开(公告)日： 1998-07-07
- 发明人: Dan W. Denney, Jr.
- 申请人： Dan W. Denney, Jr.
- 申请人地址： CA San Francisco
- 专利权人： Genitope Corporation
- 当前专利权人： Genitope Corporation
- 当前专利权人地址： CA San Francisco
- 主分类号： A61K39/00
- IPC分类号： A61K39/00 ; A61P35/00 ; C07K14/52 ; C07K14/54 ; C07K14/705 ; C07K14/725 ; C07K14/74 ; C07K16/00 ; C12N9/00 ; C12N9/06 ; C12N15/13 ; C12N15/85 ; C12P21/08 ; C12N5/10 ; C12N15/69 ; C12N15/87
摘要：
The present invention provides improved methods for the amplification and expression of recombinant genes in cells. The methods of the present invention permit the isolation of cell lines which have co-amplified input recombinant sequences which encode an amplifiable marker, one or more expression vectors encoding a protein of interest and optionally a selectable marker. The present methods allow the efficient isolation of amplified cell lines which express the protein(s) of interest in a relatively short period of time. The present invention also provides compositions comprising amplified T lymphoid cell lines.
公开/授权文献
- US5068296A Metathesis polymerized cross-linked copolymer 公开/授权日：1991-11-26
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