发明授权
- 专利标题： Method for mass production of taxol by semi-continuous culture with Taxus chinensis cell culture
- 专利标题（中）： 紫杉醇细胞培养半连续培养紫杉醇大量生产方法
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申请号： US652492申请日： 1996-05-28
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公开(公告)号： US5871979A公开(公告)日： 1999-02-16
- 发明人: Hyung-Kyoon Choi , Tom Lee Adams , Roy William Stahlhut , Sang-Ic Kim , Jeong-Hwan Yun , Bong-Kyu Song , Jin-Hyun Kim , Jun-Seog Song , Seung-Suh Hong , Hyun-Soo Lee
- 申请人： Hyung-Kyoon Choi , Tom Lee Adams , Roy William Stahlhut , Sang-Ic Kim , Jeong-Hwan Yun , Bong-Kyu Song , Jin-Hyun Kim , Jun-Seog Song , Seung-Suh Hong , Hyun-Soo Lee
- 申请人地址： KRX Seoul
- 专利权人： Samyang Genex Co., Ltd.
- 当前专利权人： Samyang Genex Co., Ltd.
- 当前专利权人地址： KRX Seoul
- 优先权： KRX95-10204 19950427
- 主分类号： C12P15/00
- IPC分类号： C12P15/00 ; C07D305/14 ; C12P17/02 ; C12R1/91
摘要：
The present invention relates to a method for mass production of taxol by semi-continuous culture of Taxus genus plant with a high yield. According to the present invention, taxol can be prepared with a high yield, by employing semi-continuous culture of Taxus genus plant cell, which comprises: (i) inoculating Taxus genus plant cell on a medium containing 1 to 10% (w/v) sugar, and incubating it; and, (ii) transferring 1/10 to 1/2 volume of the culture obtained in the step (i) to a fresh medium and repeating the step (i), adding 1 to 10% (w/v) sugar to the remnant culture and incubating to the time of maximum production of taxol.
公开/授权文献
- US5271422A Safety walker 公开/授权日：1993-12-21
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