发明授权
- 专利标题: Internal positive controls for nucleic acid amplification
- 专利标题(中): 核酸扩增的内部阳性对照
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申请号: US475283申请日: 1995-06-07
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公开(公告)号: US5882857A公开(公告)日: 1999-03-16
- 发明人: Linda M. Western , Samuel J. Rose , Edwin F. Ullman
- 申请人: Linda M. Western , Samuel J. Rose , Edwin F. Ullman
- 申请人地址: DEX Marburg
- 专利权人: Behringwerke AG
- 当前专利权人: Behringwerke AG
- 当前专利权人地址: DEX Marburg
- 主分类号: C12N15/09
- IPC分类号: C12N15/09 ; C12Q1/68 ; C07H21/02 ; C07H21/04 ; C12P19/34
摘要:
The present invention relates to an improvement in a method for amplifying a target sequence of a target polynucleotide. The method comprises combining a sample suspected of containing the target polynucleotide with reagents for amplifying the target sequence and subjecting the combination to conditions wherein the target sequence if present is amplified. The present improvement comprises including in the combination a control oligonucleotide and a control polynucleotide that has a sequence that is hybridizable with the control oligonucleotide. When the control oligonucleotide is bound to the control polynucleotide, the ability of a primer to chain extend along the control polynucleotide is reduced. Optionally, the control oligonucleotide is part of the control polynucleotide. The method finds particular application in the area of nucleic acid amplification and detection.
公开/授权文献
- US4983140A Backless, strapless ladies' body briefer 公开/授权日:1991-01-08
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