发明授权
US5922599A Method for large-scale propagation of trees of genus Gmelina by tissue
culture
失效
通过组织培养大规模繁殖Gmelina属树的方法
- 专利标题: Method for large-scale propagation of trees of genus Gmelina by tissue culture
- 专利标题(中): 通过组织培养大规模繁殖Gmelina属树的方法
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申请号: US810670申请日: 1997-03-03
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公开(公告)号: US5922599A公开(公告)日: 1999-07-13
- 发明人: Kentaro Nakamura , Ryo Soda
- 申请人: Kentaro Nakamura , Ryo Soda
- 申请人地址: JPX Osaka
- 专利权人: Sumitomo Forestry Co., Ltd.
- 当前专利权人: Sumitomo Forestry Co., Ltd.
- 当前专利权人地址: JPX Osaka
- 主分类号: A01H4/00
- IPC分类号: A01H4/00 ; C12N5/00
摘要:
Plantlets of trees of genus Gmelina can be efficiently regenerated on a large scale by culturing shoot apexes of trees of genus Gmelina on a B5 medium or its modified medium, each containing benzylaminopurine (BAP) or indole-3-butyric acid (IBA) together with BAP as plant growth regulators, thereby inducing and proliferating multiple shoots each having a large number of either definite buds or adventitious buds or both, then producing a large number of shoots from the resulting multiple shoots, transplanting the shoots to a B5 medium or its modified medium containing no plant growth regulator or containing both BAP and IBA as plant growth regulators; and the regenerated plantlets can be efficiently acclimatized to outdoor conditions by transplanting the regenerated plantlets to a mixture of river sands and crushed brick pieces in a mixing ratio of 1:1 by volume and acclimatizing the transplanted plantlets to outdoor conditions in an acclimatization box; and in this manner, trees of genus Gmelina can be propagated on a large scale.
公开/授权文献
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