A ladder hose retainer 10 for use with a ladder 48 having a ladder rung 50, includes an elongated tubular member 12 adapted to fit within a rung 50, and having a first and a second externally threaded end, 14 and 16. An internally threaded fitting 24 threadedly engages first end 14 and incorporates at least one gripping member 30 such that each gripping member 30 is adapted and arranged to securely engage a hose 58. An internally threaded cap member 22, adapted to threadedly engage second end 16, provides for adjustable mounting of the elongated member 12 within rungs 50 of various sizes. The ladder hose retainer 10 allows the secure retention of a vibrating hose 58 of a spray device to a ladder, thus reducing the vibration and discomfort imparted to an operator by the vibrating spray hose.