A power take-off release mechanism for automatically disengaging a power take-off unit and accessory equipment powered thereby is disclosed. The power take-off release mechanism interconnects a clutch foot pedal mechanism of a vehicle having a manual transmission to a throw out arm on the power take-off unit. The power take-off unit includes a mechanical cable linkage which functions to engage the power take-off unit in a conventional manner. Thereafter, whenever the manual clutch foot pedal is depressed to put the vehicle in gear under normal driving conditions, the power take-of mechanism of the present invention is automatically actuated to disengage the power take-off unit thereby preventing the same from being operated at higher engine revolutions under normal driving conditions. In one embodiment, the power take-off release mechanism includes a mechanical cable linkage that interconnects the clutch foot pedal with the mechanical throw out arm on the power take off unit. In an alternative embodiment, the same functions are carried out by electro-mechanical components such as switches and solenoids.