发明授权
- 专利标题： Modified carboxypeptidase
- 专利标题（中）： 改性羧肽酶
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申请号： US807263申请日： 1997-02-28
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公开(公告)号： US5985627A公开(公告)日： 1999-11-16
- 发明人: Uffe Mortensen , Kjeld Olesen , Henning Stennicke , Steen B. S.o slashed.rensen , Klaus Breddam
- 申请人： Uffe Mortensen , Kjeld Olesen , Henning Stennicke , Steen B. S.o slashed.rensen , Klaus Breddam
- 申请人地址： DKX Copenhagen Valby
- 专利权人： Carlsberg Laboratory
- 当前专利权人： Carlsberg Laboratory
- 当前专利权人地址： DKX Copenhagen Valby
- 主分类号： C12N9/48
- IPC分类号： C12N9/48 ; C12P13/02 ; C12N9/14
摘要：
A method for transamidating a peptide substrate having a P.sub.1 amino acid residue with a positively charged side chain. According to the invention, carboxypeptidase Y is modified to substitute at least one amino acid having a negatively charged side chain in an S.sub.1 subsite. Additionally, the modified carboxypeptidase Y can include substituted amino acid residues in an S.sub.1 ', S.sub.2 and/or S.sub.3 subsite to accommodate a specific peptide substrate.