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18 条记录 (耗时 0.022 秒)

    Customized proteases
    3.
    发明授权
    Customized proteases 失效
    定制蛋白酶

    公开(公告)号：US06187579B1

    公开(公告)日：2001-02-13

    申请号：US08329892

    申请日：1994-10-27

    申请人： Klaus Breddam ,  Morten C. Kielland-Brandt ,  Uffe Hasbo Mortensen ,  Kjeld Ove Olesen ,  Henning Ralf Stennicke ,  Fred W. Wagner

    发明人： Klaus Breddam ,  Morten C. Kielland-Brandt ,  Uffe Hasbo Mortensen ,  Kjeld Ove Olesen ,  Henning Ralf Stennicke ,  Fred W. Wagner

    IPC分类号： C12N960

    CPC分类号： C12N15/102 ,  C07K14/60 ,  C12N9/48 ,  C12N15/10 ,  C12P21/02

    摘要： The invention provides customized proteases (i.e., mutant enzymes), methods of making customized proteases, as well as methods of using customized proteases. The customized proteases of the invention are derived from the known proteases. Altered transacylation reactions include the capability to perform transacylation reactions not substantially catalyzed by the known protease or the capability to perform transacylation reactions with improved yields, or both. The methods of the invention provide for customized proteases through site specific or random mutagenesis of the active site amino acids of the known proteases. The invention also provides for methods of using the customized proteases to prepare a preselected transacylation products. The preselected transacylation products produced can be modified by substitution at the N-or C-terminal with nucleophiles such as L-amino acids, D-amino acids, amino acid amides, and radioactive amino acids.

    摘要翻译： 本发明提供定制的蛋白酶（即突变酶），制备定制蛋白酶的方法以及使用定制的蛋白酶的方法。 本发明的定制的蛋白酶来自已知的蛋白酶。 改变的转酰基反应包括执行基本上不被已知蛋白酶催化的转酰基化反应的能力或者以提高的产量进行转酰化反应的能力，或两者都有。 本发明的方法通过已知蛋白酶的活性位点氨基酸的位点特异性或随机诱变提供定制的蛋白酶。 本发明还提供使用定制的蛋白酶制备预选的转酰胺产物的方法。 所产生的预选择的转酰胺化产物可以通过用亲核试剂如L-氨基酸，D-氨基酸，氨基酸酰胺和放射性氨基酸在N-或C-末端取代来修饰。

    Barley with reduced lipoxygenase activity and beverage prepared therefrom
    6.
    发明授权
    Barley with reduced lipoxygenase activity and beverage prepared therefrom 有权
    具有降低的脂氧合酶活性的大麦和由其制备的饮料

    公开(公告)号：US09363959B2

    公开(公告)日：2016-06-14

    申请号：US13141579

    申请日：2009-12-28

    申请人： Birgitte Skadhauge ,  Finn Lok ,  Klaus Breddam ,  Ole Olsen ,  Lette Mølskov Bech ,  Søren Knudsen

    发明人： Birgitte Skadhauge ,  Finn Lok ,  Klaus Breddam ,  Ole Olsen ,  Lette Mølskov Bech ,  Søren Knudsen

    IPC分类号： A01H5/10 ,  C12C1/18 ,  C12C12/00

    CPC分类号： A01H5/10 ,  C12C1/18 ,  C12C12/00 ,  C12C2200/01

    摘要： According to the invention, there is provided barley with total loss of functional lipoxygenase (LOX)-1 and LOX-2 enzymes, and plant products produced thereof, such as malt manufactured by using barley kernels defective in the synthesis of the fatty acid-dioxygenating enzymes LOX-1 and LOX-2. Said enzymes account for the principal activities related to dioxygenation of linoleic acid into 9- and 13-hydroperoxy octadecadienoic acid, respectively. 9-Hydroperoxy octadecadienoic acid represents a LOX pathway metabolite, which—through further enzymatic or spontaneous reactions—may lead to the appearance of trans-2-nonenal (T2N). The invention enables brewers to produce a beer having insignificant levels of stale, T2N-specific off-flavors, even after prolonged storage of the beverage.

    摘要翻译： 根据本发明，提供了具有功能性脂氧合酶（LOX）-1和LOX-2酶的总损失的大麦及其生产的植物产品，例如通过使用在合成脂肪酸 - 双氧合中缺陷的大麦仁制备的麦芽 酶LOX-1和LOX-2。 所述酶分别涉及与亚油酸双加氧相关的9-和13-氢过氧十八碳二烯酸的主要活性。 9-氢过氧十八碳二烯酸代表LOX途径代谢物，通过进一步的酶促或自发反应，可能导致反式-2-壬醛（T2N）的出现。 本发明使酿造商能够生产具有不明显水平的陈年T2N特异性异味的啤酒，即使在饮料长期储存之后。

    Customized proteases
    8.
    发明授权
    Customized proteases 失效
    定制蛋白酶

    公开(公告)号：US5945329A

    公开(公告)日：1999-08-31

    申请号：US899324

    申请日：1997-07-23

    申请人： Klaus Breddam ,  Morten C. Kielland-Brandt ,  Uffe Hasbo Mortensen ,  Kjeld Ove Olesen ,  Henning Ralf Stennicke ,  Fred W. Wagner

    发明人： Klaus Breddam ,  Morten C. Kielland-Brandt ,  Uffe Hasbo Mortensen ,  Kjeld Ove Olesen ,  Henning Ralf Stennicke ,  Fred W. Wagner

    IPC分类号： C12N15/09 ,  C07K14/60 ,  C12N9/48 ,  C12N9/50 ,  C12N15/10 ,  C12N15/57 ,  C12P21/02 ,  C12R1/865 ,  C12N9/60 ,  C12N15/11 ,  C12P21/00

    CPC分类号： C12N15/102 ,  C07K14/60 ,  C12N15/10 ,  C12N9/48 ,  C12P21/02

    摘要： The invention provides customized proteases (i.e., mutant enzymes), methods of making customized proteases, as well as methods of using customized proteases. The customized proteases of the invention are derived from the known proteases. Altered transacylation reactions include the capability to perform transacylation reactions not substantially catalyzed by the known protease or the capability to perform transacylation reactions with improved yields, or both. The methods of the invention provide for customized proteases through site specific or random mutagenesis of the active site amino acids of the known proteases. The invention also provides for methods of using the customized proteases to prepare a preselected transacylation products. The preselected transacylation products produced can be modified by substitution at the N- or C-terminal with nucleophiles such as L-amino acids, D-amino acids, amino acid amides, and radioactive amino acids.

    摘要翻译： 本发明提供定制的蛋白酶（即突变酶），制备定制蛋白酶的方法以及使用定制的蛋白酶的方法。 本发明的定制的蛋白酶来自已知的蛋白酶。 改变的转酰基反应包括执行基本上不被已知蛋白酶催化的转酰基化反应的能力或者以提高的产量进行转酰化反应的能力，或两者都有。 本发明的方法通过已知蛋白酶的活性位点氨基酸的位点特异性或随机诱变提供定制的蛋白酶。 本发明还提供使用定制的蛋白酶制备预选的转酰胺产物的方法。 产生的预选择的转酰胺产物可以通过用亲核试剂例如L-氨基酸，D-氨基酸，氨基酸酰胺和放射性氨基酸在N-或C-末端取代来修饰。

    Modified carboxypeptidase
    10.
    发明授权
    Modified carboxypeptidase 失效
    改性羧肽酶

    公开(公告)号：US5985627A

    公开(公告)日：1999-11-16

    申请号：US807263

    申请日：1997-02-28

    申请人： Uffe Mortensen ,  Kjeld Olesen ,  Henning Stennicke ,  Steen B. S.o slashed.rensen ,  Klaus Breddam

    发明人： Uffe Mortensen ,  Kjeld Olesen ,  Henning Stennicke ,  Steen B. S.o slashed.rensen ,  Klaus Breddam

    IPC分类号： C12N9/48 ,  C12P13/02 ,  C12N9/14

    CPC分类号： C12N9/48

    摘要： A method for transamidating a peptide substrate having a P.sub.1 amino acid residue with a positively charged side chain. According to the invention, carboxypeptidase Y is modified to substitute at least one amino acid having a negatively charged side chain in an S.sub.1 subsite. Additionally, the modified carboxypeptidase Y can include substituted amino acid residues in an S.sub.1 ', S.sub.2 and/or S.sub.3 subsite to accommodate a specific peptide substrate.

    摘要翻译： 一种具有带正电侧链的具有P1氨基酸残基的肽底物的酰胺化方法。 根据本发明，羧肽酶Y被修饰以在S1子位点中替代具有带负电荷侧链的至少一个氨基酸。 此外，修饰的羧肽酶Y可以包括S1'，S2和/或S3子位点中的取代的氨基酸残基以适应特定的肽底物。