An apparatus and method for the biopsy of tissue specimens and, more particularly, a single insertion multiple sample percutaneous biopsy apparatus and method are provided. A tip at a distal end of a support tube is introduced into a tissue mass. The support tube has a lateral opening adjacent the tip. A knife tube is disposed within the support tube and is retracted allowing the lateral opening to communicate with the tissue mass. Suction through a suction tube draws tissue into the lateral opening where it is severed placing the tissue in a pusher. Pusher is attached to vacuum tube which is retracted proximally through the knife tube. A tissue sample is drawn through the knife tube to a location where it can be removed from the pusher.