Disclosed is a novel anti-reflective film used for reducing the adverse influences of stray light within an optical instrument such as photographic cameras by attaching onto the inner walls of the instrument. The anti-reflective film consists of a substrate film of a synthetic resin and an anti-reflective coating layer formed on one surface of the substrate film by coating with a coating composition comprising (a) a synthetic resin as a binder to form the matrix phase of the coating layer, (b) particles of a synthetic resin having an average particle diameter in the range from 15 to 50 .mu.m dispersed in the matrix phase, and (c) particles of carbon having an average particle diameter not exceeding 2 .mu.m dispersed in the matrix phase.