发明授权
- 专利标题： Controlled reversible poration for preservation of biological materials
- 专利标题（中）： 用于保存生物材料的可控可逆定点
-
申请号： US151821申请日： 1998-09-11
-
公开(公告)号： US6127177A公开(公告)日： 2000-10-03
- 发明人: Mehmet Toner , Michael Russo , Robert Bieganski
- 申请人： Mehmet Toner , Michael Russo , Robert Bieganski
- 申请人地址： MA Boston MA Boston
- 专利权人： Massachusetts Institute of Technology,The General Hospital Corporation
- 当前专利权人： Massachusetts Institute of Technology,The General Hospital Corporation
- 当前专利权人地址： MA Boston MA Boston
- 主分类号： A01N1/02
- IPC分类号： A01N1/02 ; C12N5/00
摘要：
A preservation method for biological material having cell membranes includes reversibly porating the cell membranes; loading a bio-protective agent having bio-preservation properties to a predetermined intracellular concentration; preparing the bio-protective agent loaded biological material for storage; storing the biological material; recovering the stored biological material from storage; and reversing the cell membrane poration. H5 .alpha.-toxin, a genetically engineered mutant of Staphylococcus aureus .alpha.-hemolysin, may be used as a porating agent. Non-permeating sugars such as trehalose and sucrose may be used as the bio-protective agent.
信息查询
