发明授权
- 专利标题： Methods and compositions for secretion of heterologous polypeptides
- 专利标题（中）： 用于分泌异源多肽的方法和组合物
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申请号： US08397303申请日： 1995-03-01
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公开(公告)号： US06242177B1公开(公告)日： 2001-06-05
- 发明人: Laura C. Simmons , Daniel G. Yansura
- 申请人： Laura C. Simmons , Daniel G. Yansura
- 主分类号： C12Q168
- IPC分类号： C12Q168
摘要：
The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.