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公开(公告)号：US5747662A
公开(公告)日：1998-05-05
申请号：US398617
申请日：1995-03-01
IPC分类号： C07K14/245 , C12N15/70 , C07H21/04
CPC分类号： C07K14/245 , C12N15/70
摘要： The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.
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公开(公告)号：US06242177B1
公开(公告)日：2001-06-05
申请号：US08397303
申请日：1995-03-01
IPC分类号： C12Q168
CPC分类号： C12N15/625 , C07K2319/02 , C07K2319/036 , C07K2319/61 , C07K2319/75 , C12N15/70
摘要： The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.
摘要翻译： 本发明公开了意想不到的结果，与野生型信号序列相比，具有降低的翻译强度的突变信号序列提供了基本上完整的处理和高水平的感兴趣的多肽的表达，并且许多哺乳动物多肽需要较窄的翻译水平范围 达到最大分泌。 一组信号序列载体提供一系列翻译强度，以优化感兴趣的多肽的表达。
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3.发明授权Separate-cistron contructs for secretion of aglycosylated antibodies from prokaryotes 有权分离顺式构建用于从原核生物分泌糖基化抗体公开(公告)号：US06979556B2
公开(公告)日：2005-12-27
申请号：US10020786
申请日：2001-12-13
IPC分类号： C07K16/00 , C07K16/22 , C07K16/28 , C07K16/32 , C07K16/42 , C12N1/21 , C12N15/09 , C07H21/04 , C12N15/00 , C12P21/06
CPC分类号： C07K16/36 , A61K2039/505 , C07K16/00 , C07K16/22 , C07K16/2845 , C07K16/2878 , C07K16/2896 , C07K16/32 , C07K16/4291 , C07K2317/20 , C07K2317/41 , C07K2317/52 , C07K2317/55 , C07K2319/00 , C12N15/70
摘要： The present invention provides methods and compositions for improved expression and production of recombinant antibodies in prokaryotic expression systems. Particularly contemplated are prokaryotic expression and production of full length aglycosylated antibodies. The antibody products of the invention can be used in various aspects of biological research, diagnosis and medical treatment.
摘要翻译： 本发明提供用于改进原核表达系统中重组抗体表达和产生的方法和组合物。 特别考虑的是全长糖基化抗体的原核表达和产生。 本发明的抗体产品可用于生物研究，诊断和治疗的各个方面。
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公开(公告)号：US5840523A
公开(公告)日：1998-11-24
申请号：US398615
申请日：1995-03-01
IPC分类号： C12N15/09 , C07K14/245 , C12N1/21 , C12N15/62 , C12N15/67 , C12N15/70 , C12P21/00 , C12P21/02 , C12R1/19 , C07H21/04 , C12Q1/68
CPC分类号： C07K14/245 , C12N15/625 , C12N15/70 , C07K2319/02 , C07K2319/61
摘要： The instant invention discloses the unexpected result that mutant signal sequences with reduced translational strength provided essentially complete processing and high levels of expression of a polypeptide of interest as compared to wild type signal sequences, and that many mammalian polypeptides require a narrow range of translation levels to achieve maximum secretion. A set of signal sequence vectors provides a range of translational strengths for optimizing expression of a polypeptide of interest.
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公开(公告)号：US20110111408A1
公开(公告)日：2011-05-12
申请号：US12940825
申请日：2010-11-05
CPC分类号： C07K16/30 , C07K16/00 , C07K2317/14 , C07K2319/02
摘要： The present invention relates generally to the fields of molecular biology and protein technology. More specifically, the invention concerns signal sequences for the secretion of heterologous polypeptide from bacteria. The invention also concerns recombinant polypeptides and uses thereof.
摘要翻译： 本发明一般涉及分子生物学和蛋白质技术领域。 更具体地，本发明涉及用于从细菌中分泌异源多肽的信号序列。 本发明还涉及重组多肽及其用途。
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