发明授权
US06245545B1 Method for cloning and producing the SwaI restriction endonuclease
有权
用于克隆和产生SwaI限制性内切核酸酶的方法
- 专利标题： Method for cloning and producing the SwaI restriction endonuclease
- 专利标题（中）： 用于克隆和产生SwaI限制性内切核酸酶的方法
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申请号： US09299378申请日： 1999-04-27
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公开(公告)号： US06245545B1公开(公告)日： 2001-06-12
- 发明人: Huimin Kong , Lauren S. Higgins , Michael A. Dalton
- 申请人： Huimin Kong , Lauren S. Higgins , Michael A. Dalton
- 主分类号： C12N922
- IPC分类号： C12N922
摘要：
The present invention relates to the recombinant DNA which encodes the SwaI restriction endonuclease, modification methylase, and the production of SwaI restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.
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