-
1.发明授权公开(公告)号：US06245545B1
公开(公告)日：2001-06-12
申请号：US09299378
申请日：1999-04-27
IPC分类号： C12N922
CPC分类号： C12N9/22
摘要： The present invention relates to the recombinant DNA which encodes the SwaI restriction endonuclease, modification methylase, and the production of SwaI restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.
摘要翻译： 本发明涉及编码SwaI限制性内切核酸酶，修饰甲基化酶和从重组DNA产生SwaI限制性内切核酸酶的重组DNA。 还公开了相关表达载体pHKUV5，其特征在于不含Lac阻遏物结合位点的强的组成型UV5启动子和含有强大的可控T7启动子和低拷贝数复制起点的pHKT7。
-
2.发明授权
公开(公告)号：US6048719A
公开(公告)日：2000-04-11
申请号：US235246
申请日：1999-01-22
CPC分类号： C12N9/22
摘要： The present invention relates to the recombinant DNA which encodes the DraIII restriction endonuclease modification methylase, and the production of DraIII restriction endonuclease from the recombinant DNA. Related expression vectors, pHKUV5 vector which features a strong, constitutive UV5 promoter without the Lac repressor binding site and pHKT7 vector which contains a powerful controllable T7 promoter and a low copy number origin of replication, are also disclosed.
摘要翻译： 本发明涉及编码DraIII限制性内切核酸酶修饰甲基化酶的重组DNA，以及从重组DNA产生DraIII限制性内切核酸酶。 还公开了相对表达载体，其具有强的组成型UV5启动子的pHKUV5载体，其不含Lac阻遏物结合位点和含有强大的可控T7启动子和低拷贝数复制起点的pHKT7载体。
-
3.发明授权
公开(公告)号：US6048731A
公开(公告)日：2000-04-11
申请号：US146675
申请日：1998-09-03
CPC分类号： C12N9/22
摘要： The present invention relates to the recombinant DNA which encodes the SgrAI restriction endonuclease and the MspI modification methylase, and the production of SgrAI restriction endonuclease from the recombinant DNA.
摘要翻译： 本发明涉及编码SgrAI限制性内切核酸酶和MspI修饰甲基化酶的重组DNA，以及从重组DNA产生SgrAI限制性内切核酸酶。
-
公开(公告)号：US06191267B1
公开(公告)日：2001-02-20
申请号：US09586935
申请日：2000-06-02
IPC分类号： C07H2104
摘要： The present invention relates to recombinant DNA which encodes a novel nicking endonuclease, N.BstNBI, and the production of N.BstNBI restriction endonuclease from the recombinant DNA utilizing PleI modification methylase. Related expression vectors, as well as the application of N.BstNBI in non-thio strand displacement amplification, is disclosed also.
摘要翻译： 本发明涉及编码新的切口内切核酸酶，N.BstNBI，以及利用PleI修饰甲基化酶从重组DNA生产N.BstNBI限制性内切核酸酶的重组DNA。 相关表达载体以及N.BstNBI在非硫链置换扩增中的应用也被公开。
-
公开(公告)号：US20060154286A1
公开(公告)日：2006-07-13
申请号：US11327175
申请日：2006-01-05
申请人： Huimin Kong , Myriam Vincent , Yan Xu , Wen Tang
发明人： Huimin Kong , Myriam Vincent , Yan Xu , Wen Tang
CPC分类号： C12P19/34 , C12N15/1096
摘要： Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a single strand helicase preparation or a thermostable helicase in the absence of a single strand binding protein and a DNA polymerase such that the amplification can be performed isothermally.
摘要翻译： 提供了用于选择性和指数扩增核酸的方法和试剂盒，并且包括在不存在单链结合蛋白和DNA聚合酶的情况下使用单链解旋酶制剂或热稳定解旋酶，使得可以等温进行扩增。
-
6.发明授权Method for cloning and expression of PleI restriction endonuclease and PleI and BstNBII methylases in E. coli 失效克隆和表达大肠杆菌中PleI限制性内切核酸酶和PleI和BstNBII甲基化酶的方法公开(公告)号：US06391608B1
公开(公告)日：2002-05-21
申请号：US09813872
申请日：2001-03-21
申请人： Huimin Kong , Lauren Sears Higgins
发明人： Huimin Kong , Lauren Sears Higgins
IPC分类号： C12N922
CPC分类号： C12N9/22 , C12N9/1007
摘要： The present invention relates to recombinant DNA which encodes the PleI restriction endonuclease as well as PleI and BstNBII methyltransferase and expression of PleI restriction endonuclease and M.BstNBII in E. coli cells containing the recombinant DNA.
摘要翻译： 本发明涉及编码PleI限制性内切核酸酶以及PleI和BstNBII甲基转移酶的重组DNA，以及含有重组DNA的大肠杆菌细胞中PleI限制性内切核酸酶和M.BstNBII的表达。
-
公开(公告)号：US08685648B2
公开(公告)日：2014-04-01
申请号：US13146602
申请日：2010-02-03
申请人： Huimin Kong , Yanhong Tong
发明人： Huimin Kong , Yanhong Tong
IPC分类号： C12Q1/68
CPC分类号： C12Q1/6844 , C12P19/34 , C12Q1/686 , C12Q2521/531 , C12Q2521/301
摘要： The invention provides methods and compositions for enhancing the speed and sensitivity of helicase-dependent amplification through the use of an endonuclease.
摘要翻译： 本发明提供了通过使用内切核酸酶增强解旋酶依赖性扩增的速度和灵敏度的方法和组合物。
-
8.发明申请公开(公告)号：US20110229887A1
公开(公告)日：2011-09-22
申请号：US12962202
申请日：2010-12-07
申请人： Huimin Kong , Yanhong Tong , Wen Tang
发明人： Huimin Kong , Yanhong Tong , Wen Tang
IPC分类号： C12Q1/68 , G01N33/543
CPC分类号： C12Q1/6834 , G01N33/54386 , C12Q2545/101 , C12Q2565/513
摘要： Compositions and methods useful in nucleic acid assays are provided. The invention permits detection of test and control nucleic acids. Test nucleic acids can be immobilized at multiple locations, such that amplification of either a test nucleic acid or a control nucleic acid provides a captured nucleic acid in a control capture zone.
摘要翻译： 提供了可用于核酸测定的组合物和方法。 本发明允许检测和控制核酸。 测试核酸可以在多个位置被固定，使得测试核酸或对照核酸的扩增在捕获区域中提供捕获的核酸。
-
公开(公告)号：US07829284B2
公开(公告)日：2010-11-09
申请号：US11796675
申请日：2007-04-27
申请人： Huimin Kong , Myriam Vincent , Yan Xu
发明人： Huimin Kong , Myriam Vincent , Yan Xu
CPC分类号： C12Q1/6844 , C12Q2521/513
摘要： Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.
摘要翻译： 提供了用于选择性和指数扩增核酸的方法和试剂盒，并且包括使用解旋酶制备物和DNA聚合酶，使得扩增可以等温进行。
-
公开(公告)号：US20070207495A1
公开(公告)日：2007-09-06
申请号：US11796675
申请日：2007-04-27
申请人： Huimin Kong , Myriam Vincent , Yan Xu
发明人： Huimin Kong , Myriam Vincent , Yan Xu
CPC分类号： C12Q1/6844 , C12Q2521/513
摘要： Methods and a kit are provided for selectively and exponentially amplifying nucleic acids and include the use of a helicase preparation and a DNA polymerase such that the amplification can be performed isothermally.
-
-
-
-
-
-
-
-
-
搜索结果
21 条记录 (耗时 0.024 秒)
