发明授权
- 专利标题: Hydrolyzing fats and oils using an immobilized enzyme column and substrate-feeding chamber that separates phases
- 专利标题(中): 使用分离相的固定化酶柱和底物进料室水解脂肪和油
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申请号: US09448140申请日: 1999-11-24
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公开(公告)号: US06258575B1公开(公告)日: 2001-07-10
- 发明人: Masami Shimizu , Toshiteru Komatsu , Naoto Yamada
- 申请人: Masami Shimizu , Toshiteru Komatsu , Naoto Yamada
- 优先权: JP10-335164 19981126; JP11-106607 19990414
- 主分类号: C12P764
- IPC分类号: C12P764
摘要:
An oil or fat phase is provided above an aqueous phase in a substrate-feeding chamber such that there is a distinct interface between the phases. The phases are separately removed from the chamber, mixed together and fed to an immobilized-enzyme reaction column to produce a reaction mixture. The mixture is fed to the substrate-feeding chamber and introduced into the oil or fat phase such that the interface remains distinct and undisturbed, and monoglycerides, diglycerides and fatty acids remain in the oil or fat phase and water and glycerol separate into the aqueous phase. Steps of the process are repeated while maintaining the interface distinct and undisturbed in the substrate-feeding chamber until a desired degree of oil or fat hydrolysis is obtained. The mixture from the reaction column may be fed to a mixing chamber, mixed with water from the substrate-feeding chamber, and fed to the substrate-feeding chamber. In another embodiment, only the oil or fat phase is fed to the reaction column to produce a reaction mixture that is fed to the mixing chamber and then to the substrate-feeding chamber. The process provides a high degree of hydrolysis due to adequate extraction of glycerol.
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