发明授权
US06258607B1 Indirect agglutination immunoassay and apparatus therefor 失效
间接凝集免疫测定及其设备

  • 专利标题: Indirect agglutination immunoassay and apparatus therefor
  • 专利标题(中): 间接凝集免疫测定及其设备
  • 申请号: US08082373
    申请日: 1993-06-28
  • 公开(公告)号: US06258607B1
    公开(公告)日: 2001-07-10
  • 发明人: Tomo SaitoMikio Ikeda
  • 申请人: Tomo SaitoMikio Ikeda
  • 优先权: JP1-281895 19891031; JP1-329556 19891221
  • 主分类号: G01N33553
  • IPC分类号: G01N33553
Indirect agglutination immunoassay and apparatus therefor
摘要:
An indirect agglutination immunoassay includes the steps of providing, in a container, an immunoassay system comprising a test sample containing a desired analyte, and a reagent composed of magnetic particles or magnetic-material containing particles containing iron therein, wherein the magnetic particles or magnetic-material containing particles have been sensitized to allow specific binding to the desired analyte, and have a particle size in the range of 1 to 5 &mgr;m, with the content of the iron being in the range of 8 to 20 wt. %, precipitating the magnetic particles or magnetic-material containing particles by the application of magnetic force, allowing the container to stand at an inclination, and detecting the presence or absence of an immune reaction from the absence or presence of slippage observed of the precipitated magnetic particles or magnetic-material containing particles on the bottom of the container. An apparatus for conducting this indirect agglutination immunoassay is composed of the above container, a magnetic sedimentation device for magnetically precipitating the components containing the magnetic particles at the bottom of the container, and an inclination device for allowing the container to stand at an inclination after removal of the magnetic sedimentation device.
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