发明授权
US06268411B1 Use of multivalent chimeric peptide-loaded, MHC/ig molecules to detect, activate or suppress antigen-specific T cell-dependent immune responses
有权
使用多价嵌合肽加载的MHC / ig分子检测，激活或抑制抗原特异性T细胞依赖性免疫应答
- 专利标题： Use of multivalent chimeric peptide-loaded, MHC/ig molecules to detect, activate or suppress antigen-specific T cell-dependent immune responses
- 专利标题（中）： 使用多价嵌合肽加载的MHC / ig分子检测，激活或抑制抗原特异性T细胞依赖性免疫应答
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申请号： US09150622申请日： 1998-09-10
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公开(公告)号： US06268411B1公开(公告)日： 2001-07-31
- 发明人: Jonathan Schneck , Drew Pardoll , Sean O'Herrin , Jill Slansky , Tim Greten
- 申请人： Jonathan Schneck , Drew Pardoll , Sean O'Herrin , Jill Slansky , Tim Greten
- 主分类号： C07K16100
- IPC分类号： C07K16100
摘要：
To increase the effective affinity of soluble analogs of peptide/MHC molecules for their cognate ligands, divalent peptide/MHC complexes were constructed. Using a recombinant DNA strategy, DNA encoding the MHC class I was ligated to DNA coding for murine Ig heavy chain. MHC/Ig complexes were exploited to homogeneously load with peptides of interest. The results of flow cytometry demonstrated that the pepMHC/Ig complexes bound specifically with high affinity to cells bearing their cognate receptors. pepMHC/Ig complexes are also useful in modulating effector functions of antigen-specific T cells. These pepMHC/Ig complexes are useful for studying TCR/MHC interactions and lymphocyte tracking and have uses as specific regulators of immune responses.
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