公开(公告)号：US06268411B1

公开(公告)日：2001-07-31

申请号：US09150622

申请日：1998-09-10

申请人： Jonathan Schneck ,  Drew Pardoll ,  Sean O'Herrin ,  Jill Slansky ,  Tim Greten

发明人： Jonathan Schneck ,  Drew Pardoll ,  Sean O'Herrin ,  Jill Slansky ,  Tim Greten

IPC分类号： C07K16100

CPC分类号： A61K39/385 ,  A61K38/00 ,  A61K39/00 ,  A61K47/6425 ,  A61K47/6811 ,  A61K2035/124 ,  A61K2039/5154 ,  A61K2039/5158 ,  A61K2039/605 ,  A61K2039/6056 ,  C07K14/005 ,  C07K14/7051 ,  C07K14/70539 ,  C07K2319/00 ,  C07K2319/30 ,  C12N2710/14143 ,  C12N2740/13022 ,  C12N2740/14022 ,  C12N2760/16122 ,  C12N2760/16134

摘要： To increase the effective affinity of soluble analogs of peptide/MHC molecules for their cognate ligands, divalent peptide/MHC complexes were constructed. Using a recombinant DNA strategy, DNA encoding the MHC class I was ligated to DNA coding for murine Ig heavy chain. MHC/Ig complexes were exploited to homogeneously load with peptides of interest. The results of flow cytometry demonstrated that the pepMHC/Ig complexes bound specifically with high affinity to cells bearing their cognate receptors. pepMHC/Ig complexes are also useful in modulating effector functions of antigen-specific T cells. These pepMHC/Ig complexes are useful for studying TCR/MHC interactions and lymphocyte tracking and have uses as specific regulators of immune responses.

摘要翻译： 为了增加肽/ MHC分子的可溶性类似物对其同源配体的有效亲和力，构建了二价肽/ MHC复合物。 使用重组DNA策略，将编码MHC I类的DNA连接到编码鼠Ig重链的DNA上。 利用MHC / Ig复合物均匀加载感兴趣的肽。 流式细胞仪的结果表明，具有高亲和力的pepMHC / Ig复合物特异性结合携带其同源受体pepMHC / Ig复合物的细胞也可用于调节抗原特异性T细胞的效应子功能。 这些pepMHC / Ig复合物可用于研究TCR / MHC相互作用和淋巴细胞跟踪，并将其用作免疫应答的特异性调节物。

公开(公告)号：US06734013B2

公开(公告)日：2004-05-11

申请号：US09789720

申请日：2001-02-22

申请人： Jonathan Schneck ,  Drew Pardoll ,  Sean O'Herrin ,  Jill Slansky ,  Tim Greten

发明人： Jonathan Schneck ,  Drew Pardoll ,  Sean O'Herrin ,  Jill Slansky ,  Tim Greten

IPC分类号： C12N1563

CPC分类号： A61K39/385 ,  A61K38/00 ,  A61K39/00 ,  A61K47/6425 ,  A61K47/6811 ,  A61K2035/124 ,  A61K2039/5154 ,  A61K2039/5158 ,  A61K2039/605 ,  A61K2039/6056 ,  C07K14/005 ,  C07K14/7051 ,  C07K14/70539 ,  C07K2319/00 ,  C07K2319/30 ,  C12N2710/14143 ,  C12N2740/13022 ,  C12N2740/14022 ,  C12N2760/16122 ,  C12N2760/16134

摘要： To increase the effective affinity of soluble analogs of peptide/MHC molecules for their cognate ligands, divalent peptide/MHC complexes were constructed. Using a recombinant DNA strategy, DNA encoding the MHC class I was ligated to DNA coding for murine Ig heavy chain. MHC/Ig complexes were exploited to homogeneously load with peptides of interest. The results of flow cytometry demonstrated that the pepMHC/Ig complexes bound specifically with high affinity to cells bearing their cognate receptors. pepMHC/Ig complexes are also useful in modulating effector functions of antigen-specific T cells. These pepMHC/Ig complexes are useful for studying TCR/MHC interactions and lymphocyte tracking and have uses as specific regulators of immune responses.

摘要翻译： 为了增加肽/ MHC分子的可溶性类似物对其同源配体的有效亲和力，构建了二价肽/ MHC复合物。 使用重组DNA策略，将编码MHC I类的DNA连接到编码鼠Ig重链的DNA上。 利用MHC / Ig复合物均匀加载感兴趣的肽。 流式细胞术的结果表明，MHC / Ig复合物特异性结合具有高亲和力的细胞携带其同源受体。 MHC / Ig复合物也可用于调节抗原特异性T细胞的效应子功能。 这些MHC / Ig复合物可用于研究TCR / MHC相互作用和淋巴细胞跟踪，并且具有用作免疫应答的特异性调节物。