On a main surface of a p-type silicon substrate having a bipolar transistor forming region and a MOS transistor forming region, an epitaxial layer is grown and n-type buried layers are formed. After forming a trench penetrating the buried layer, a buried polysilicon layer is formed in the trench. Then, a threshold control layer, a punch-through stopper layer, a channel stopper layer, an n-type well layer and a p-type well layer of each MOSFET are formed. At this point, since the well layer is formed through high energy ion implantation, the n-type buried layer is suppressed from being enlarged, and hence, time required for forming the trench can be shortened. Thus, a practical method of manufacturing a semiconductor device is provided.