发明授权
US06368860B1 Anthocyanin producing callus line in cultures of panax sikkimensis and a method of producing panax sikkimensis line capable of producing anthocyanin
失效
花生素生产猕猴桃培养物中的花青素生产线和一种能够产生花青素的西葫芦系的生产方法
- 专利标题： Anthocyanin producing callus line in cultures of panax sikkimensis and a method of producing panax sikkimensis line capable of producing anthocyanin
- 专利标题（中）： 花生素生产猕猴桃培养物中的花青素生产线和一种能够产生花青素的西葫芦系的生产方法
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申请号： US09538425申请日： 2000-03-29
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公开(公告)号： US06368860B1公开(公告)日： 2002-04-09
- 发明人: Archana Mathur , Anita Gangwar , Ajay Kumar Mathur , Rajender Singh Sangwan , Dharam Chand Jain
- 申请人： Archana Mathur , Anita Gangwar , Ajay Kumar Mathur , Rajender Singh Sangwan , Dharam Chand Jain
- 主分类号： C12N500
- IPC分类号： C12N500
摘要：
The present invention relates to an anthocyanin producing callus line in cultures of Panax sikkimensis (an Indian species of ginseng) said line comprising: (a) Characteristic pink-purple pigmentation, (b) Growth index of about 221-450 in about 50-80 days of culture period, (c) Yield of anthocyanin (2-3 mg/gf.wt.) from the callus in about 40-60 days under light conditions (continuous light), and (d) Characteristic DNA profile wherein the lanes 1,2,3,4,5,6,7,8,9,10,11 and 12 in each gel (upper gel for wild line and lower gel for anthocyanin producing line) represent the PCR amplified fragments produced by the respective template megabase genomic DNA with primers 5′ CTG ATG CAT C3′, 3′,5′ TGG TCA CTG A 3′, 5′ AGG GGT CTT G 3′,5′ GAA ACG GGT G 3′,5′ AGG GGT CTT G 3′,5′ GCG TAA CGC C 3′,5′ CAG CAC CCA C 3′,5′ GTT GCG ATC C 3′,5′ CAG GCC CTT C 3′,5′ CGC AGT ACT C 3′,5′ GTC CTA CTC G 3′,5′ CTA CAC AGG C 3′ and 5′ GTC CTT AGC G 3′, respectively. Lane M shows the standard size markers of a ladder at 100 bp intervals from 1000 bp downwards.
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