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    Anthocyanin producing callus line in cultures of panax sikkimensis and a method of producing panax sikkimensis line capable of producing anthocyanin
    1.
    发明授权
    Anthocyanin producing callus line in cultures of panax sikkimensis and a method of producing panax sikkimensis line capable of producing anthocyanin 失效
    花生素生产猕猴桃培养物中的花青素生产线和一种能够产生花青素的西葫芦系的生产方法

    公开(公告)号：US06368860B1

    公开(公告)日：2002-04-09

    申请号：US09538425

    申请日：2000-03-29

    申请人： Archana Mathur ,  Anita Gangwar ,  Ajay Kumar Mathur ,  Rajender Singh Sangwan ,  Dharam Chand Jain

    发明人： Archana Mathur ,  Anita Gangwar ,  Ajay Kumar Mathur ,  Rajender Singh Sangwan ,  Dharam Chand Jain

    IPC分类号： C12N500

    CPC分类号： A01H4/005 ,  C12N5/04 ,  C12P23/00

    摘要： The present invention relates to an anthocyanin producing callus line in cultures of Panax sikkimensis (an Indian species of ginseng) said line comprising: (a) Characteristic pink-purple pigmentation, (b) Growth index of about 221-450 in about 50-80 days of culture period, (c) Yield of anthocyanin (2-3 mg/gf.wt.) from the callus in about 40-60 days under light conditions (continuous light), and (d) Characteristic DNA profile wherein the lanes 1,2,3,4,5,6,7,8,9,10,11 and 12 in each gel (upper gel for wild line and lower gel for anthocyanin producing line) represent the PCR amplified fragments produced by the respective template megabase genomic DNA with primers 5′ CTG ATG CAT C3′, 3′,5′ TGG TCA CTG A 3′, 5′ AGG GGT CTT G 3′,5′ GAA ACG GGT G 3′,5′ AGG GGT CTT G 3′,5′ GCG TAA CGC C 3′,5′ CAG CAC CCA C 3′,5′ GTT GCG ATC C 3′,5′ CAG GCC CTT C 3′,5′ CGC AGT ACT C 3′,5′ GTC CTA CTC G 3′,5′ CTA CAC AGG C 3′ and 5′ GTC CTT AGC G 3′, respectively. Lane M shows the standard size markers of a ladder at 100 bp intervals from 1000 bp downwards.

    摘要翻译： 本发明涉及一种在人参中培养的花青素生产愈伤组织线，其中包括：（a）特征性粉红紫色色素沉淀，（b）约501-80的生长指数约为221-450 培养时间段，（c）在光照条件（连续光照）下，约40-60天从愈伤组织中花青素（2-3mg / gf.wt。）的产量，（d）特征DNA谱，其中泳道1 ，每个凝胶中的2,3,4,5,6,7,8,9,10,11和12（用于野生型线的上凝胶和用于花青素生产线的下凝胶）代表由相应模板megabase产生的PCR扩增片段 基因组DNA与引物5'CTG ATG CAT C3'，3'，5'TGG TCA CTG A 3'，5'AGG GGT CTT G 3'，5'GAA ACG GGT G 3'，5'AGG GGT CTT G 3' ，5'GCG TAA CGC C 3'，5'CAG CAC CCA C 3'，5'GTT GCG ATC C 3'，5'CAG GCC CTT C 3'，5'CGC AGT ACT C 3'，5'GTC CTA CTC G 3'，5'CTA CAC AGG C 3'和5'GTC CTT AGC G 3'。 泳道M显示从1000bp向下以100bp间隔的梯子的标准尺寸标记。

    Process for isolation of withaferin-A from plant materials and products therefrom
    2.
    发明授权
    Process for isolation of withaferin-A from plant materials and products therefrom 有权
    从植物材料和产品中分离嗜铬蛋白A的方法

    公开(公告)号：US07108870B2

    公开(公告)日：2006-09-19

    申请号：US10822858

    申请日：2004-04-13

    申请人： Rajender Singh Sangwan ,  Narayan Das Chaurasiya ,  Laxmi Narayan Misra ,  Payare Lal ,  Girish Chandra Uniyal ,  Neelam Singh Sangwan ,  Avdhesh Kumar Srivastava ,  Krishan Avtar Suri ,  Ghulam Nabi Qazi ,  Rakesh Tuli

    发明人： Rajender Singh Sangwan ,  Narayan Das Chaurasiya ,  Laxmi Narayan Misra ,  Payare Lal ,  Girish Chandra Uniyal ,  Neelam Singh Sangwan ,  Avdhesh Kumar Srivastava ,  Krishan Avtar Suri ,  Ghulam Nabi Qazi ,  Rakesh Tuli

    IPC分类号： A61K36/00 ,  A61K31/35

    CPC分类号： A61K36/81

    摘要： The present invention relates to an improved process of analytical and quantitative isolation of withaferin-A from Withania somnifera (Sanskrit: Ashwagandha, English: winter cherry) and other plants and products therefrom, said method comprising steps of selecting most appropriate extraction medium composition, with extraction solvent system consisting of a defined admixture of water and alcohol in a range of proportion of alcohol within a narrow range of (methanol, ethanol etc.) (preferably 60% alcohol and 40% water) for the dry plant material and from 60% to 80% alcohol in the alcohol-water mixture for fresh plant material, the invented extractant composition leading to better/improved yields and accurate quantitative estimations of withaferin A existent in planta or in materia, selecting effective state of the tissue particularly use of the fresh materials improving yields, providing logistic benefits of isolations and diagnosis, and according several other advantages including technological, economic, preventing contaminations etc., the said method being valuable and advantageous for standardization of botanicals, metabolomic characterization of plants particularly chemotypes, analyzing metabolic transitions under physiological, genetical, environmental and biotic perturbations.

    摘要翻译： 本发明涉及一种分离和定量分离来自隐翅藻（梵语：Ashwagandha，英语：冬樱桃）和其他植物及其产物的咖啡因-A的改进方法，所述方法包括以下步骤：选择最合适的提取培养基组合物， 萃取溶剂体系，由干燥植物材料的（甲醇，乙醇等）（优选60％酒精和40％水）的窄范围内的醇的比例范围内的水和醇的混合物组成， 到新鲜植物材料的酒精 - 水混合物中的80％酒精，本发明的提取剂组合物导致在植物或物质中存在的更好/提高的产量和准确的定量估计，选择组织的有效状态，特别是使用新鲜的 材料提高产量，提供隔离和诊断的后勤优势，以及其他几个优势，包括技术 所述方法对于植物学的标准化，植物的代谢组学特征，特别是化学型，分析生理，遗传，环境和生物扰动下的代谢转变是有价值和有利的。

    BETA-GLUCOSIDASE AND A PROCESS FOR EXTRACTION THEREOF
    4.
    发明申请
    BETA-GLUCOSIDASE AND A PROCESS FOR EXTRACTION THEREOF 审中-公开
    β-葡萄糖苷酶及其提取方法

    公开(公告)号：US20070212745A1

    公开(公告)日：2007-09-13

    申请号：US11531032

    申请日：2006-09-12

    申请人： Neelam Singh Sangwan ,  Sidharth Kumar Misra ,  Suman Preet Singh Khanuja ,  Advadesh Kumar Srivastava ,  Rajender Singh Sangwan

    发明人： Neelam Singh Sangwan ,  Sidharth Kumar Misra ,  Suman Preet Singh Khanuja ,  Advadesh Kumar Srivastava ,  Rajender Singh Sangwan

    IPC分类号： C12Q1/34 ,  C12N9/24

    CPC分类号： C12Q1/34 ,  C12N9/2445 ,  C12Y302/01021

    摘要： The present invention provides a novel beta glucosidase and a process for extraction of a beta-glucosidase from Rauvolfia serpentine useful for the cleaving of beta-1,4 linkage of PNPG and to convert other gluco-conjugates such as strictosidine and raucaffricine into their corresponding aglycon such as vomilenine, commercially through immobilizing the enzyme. The β glucosidase enzyme has shown maximum activity in the acid pH range, with high optimum temperature using PNPG as substrate. The crude enzyme, when stored at 4° C., was quite stable for 6 days with 50% loss of activity. The enzyme was activated in presence of FeSO4 in the assay mixture.

    摘要翻译： 本发明提供了一种新颖的β-葡糖苷酶和从Rauvolfia蛇纹石中提取β-葡糖苷酶的方法，其可用于切割PNPG的β-1,4键并将其它葡糖共轭物如斯曲霉素和草酸转化成其相应的糖苷配基 如维生素E，通过固定酶进行商业化。 β-葡萄糖苷酶在酸性pH范围内显示出最大的活性，使用PNPG作为底物具有最高的最佳温度。 当粗酶在4℃下储存时，相当稳定6天，活性降低50％。 该酶在测定混合物中在FeSO 4的存在下活化。

    Protein profiling of hyper acidic plants and high protein extraction compositions thereof
    6.
    发明授权
    Protein profiling of hyper acidic plants and high protein extraction compositions thereof 失效
    高酸性植物的蛋白质分析及其高蛋白质提取组合物

    公开(公告)号：US06893667B2

    公开(公告)日：2005-05-17

    申请号：US10104062

    申请日：2002-03-22

    申请人： Rajender Singh Sangwan ,  Neelam Singh Sangwan ,  Bali Ram Tyagi ,  Avdhesh Kumar Srivastava ,  Usha Yadav

    发明人： Rajender Singh Sangwan ,  Neelam Singh Sangwan ,  Bali Ram Tyagi ,  Avdhesh Kumar Srivastava ,  Usha Yadav

    IPC分类号： C12Q1/28 ,  C12Q1/44 ,  C12Q1/52 ,  A61K35/78 ,  A01N37/18

    CPC分类号： C12Q1/52 ,  C12Q1/28 ,  C12Q1/44 ,  G01N2550/00

    摘要： The present invention relates to a method for profiling of protein extract from hyper-acidic aerial parts of plant Scented Geranium (Pelargonium sp.), said method comprising steps of selecting effective extraction medium, with extraction medium consisting of 0.2 M sodium carbonate increasing percentage extract by about 600%, selecting effective medium to determine polypeptide pattern in said extract, with polypeptide pattern medium consisting of Sodium Carbonate ranging between 0.2 to 0.4 M, and 0.2 M Sodium Carbonate along with 50% dimethylsulfoxide, selecting effective media to determine enzyme multimolecular forms in said extract, with enzyme multimolecular forms media consisting of 0.2 M Tris-HCl buffer at pH 7.5, and optionally 50% dimethylsulfoxide; Sodium Carbonate at concentration ranging between 0.2 to 0.4 M and optionally 50% dimethylsulfoxide, and using biochemical and biophysical techniques comprising gel electrophoresis, spectrophotometry, centrifugation, protein estimation in said method to determine quantitative, qualitative, structural and functional facets of the proteins from said extract, and extraction compositions thereof.

    摘要翻译： 本发明涉及一种用于从植物香味天竺葵（天竺葵属）的高酸性地上部分分析蛋白质提取物的方法，所述方法包括以下步骤：选择有效的提取介质，用0.2M碳酸钠提取百分比提取物 选择有效培养基以确定所述提取物中的多肽模式，多肽模式培养基由碳酸钠组成，范围在0.2至0.4M之间，0.2M碳酸钠与50％二甲基亚砜组成，选择有效培养基以确定酶多分子形式 在所述提取物中，用酶多分子形式的培养基由pH7.5的0.2M Tris-HCl缓冲液和任选的50％二甲基亚砜组成; 碳酸钠的浓度范围为0.2至0.4M，任选的50％的二甲基亚砜，并且使用生物化学和生物物理技术，包括凝胶电泳，分光光度法，离心，所述方法中的蛋白质估计，以确定所述蛋白质的定量，定性，结构和功能方面 提取物及其提取组合物。

    Process for the induction of normal roots on nodes and internodes of stem segments without using hormone and/or chemical treatments in mentha species
    7.
    发明授权
    Process for the induction of normal roots on nodes and internodes of stem segments without using hormone and/or chemical treatments in mentha species 失效
    在茎段的节点和节间上诱导正常根而不在薄荷物种中使用激素和/或化学处理的过程

    公开(公告)号：US06586248B2

    公开(公告)日：2003-07-01

    申请号：US09258883

    申请日：1999-03-01

    申请人： Rajender Singh Sangwan ,  Neelam Singh Sangwan ,  Sushil Kumar

    发明人： Rajender Singh Sangwan ,  Neelam Singh Sangwan ,  Sushil Kumar

    IPC分类号： C12N500

    CPC分类号： C12N5/04

    摘要： The invention provides a process for the induction of normal rooting on nodal and internodal stem segments without using hormones and/or other chemical treatments in Mentha species, which comprises oblique excision of shoots of Mentha species from the plants, immediately dipping the cut end of twigs or stems into water and keeping in complete dark for up to one week with by varying the temperature cycle for each set of 24 hours duration of the treatment.

    摘要翻译： 本发明提供了一种用于在节点和节间茎节段中诱导正常生根的方法，而不使用芒果种中的激素和/或其他化学处理，其包括从植物中倾斜切除芒塔种苗，立即浸入枝条的切端 或者通过改变每组24小时治疗持续时间的温度循环，将其浸入水中并保持在完全黑暗中长达一周。